伊德本可以通过肌细胞中的线粒体生物发生和线粒体生物生成缓解罗诺诱导的线粒体功能障碍
Chen Zhang1, Mei Li2, Liying Meng1
1Mitochondrial Medicine Laboratory, Qilu Hospital (Qingdao), Shandong University, Qingdao, Shandong 266035, China; Department of Central Laboratory, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, Shandong 266035, China.
伊德本通过减少氧化应激和改善线粒体功能来保护肌肉细胞免受轮损伤. 这种抗氧化剂可能为骨肌肉缩提供治疗策略.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 线粒体医学 线粒体医学
背景情况:
- 作为一种辅酶Q10类似物,idebenone是一种已知的抗氧化剂,用于治疗线粒体功能障碍.
- 它对罗农诱导的肌肉细胞损伤的疗效仍然未被探索.
研究的目的:
- 为了研究伊德本对C2C12神经细胞的保护作用,对罗诺诱导的线粒体功能障碍和氧化应激.
- 探索PGC1A在idebenone治疗作用中的作用.
主要方法:
- 暴露于rotenone的C2C12神经细胞,有或没有idebenone治疗.
- 评估了细胞活力,活性氧物种 (ROS) 水平,线粒体/自标志物 (PINK1,PARKIN,p62/SQSTM1),线粒体裂变 (DRP1,FIS1) 和能量产生 (海马分析).
- 研究了DRP1转位和PGC1A抑制的作用.
主要成果:
- 轮能诱导细胞死亡,增加ROS,激活线粒细胞衰变/自,并损害线粒体功能 (裂变,减少能量产生).
- 伊代诺治疗逆转了罗诺诱导的损伤,包括亡和氧化应激.
- 抑制PGC1A可以否定idebenone对线粒体生物发生和功能的有益作用.
结论:
- 伊德本有效地改善了轮诱导的亡,氧化应激和骨肌细胞中的线粒体损伤.
- 伊德本的保护机制涉及PGC1A介导的线粒体生物发生和功能.
- 伊德本显示了与线粒体功能障碍相关的骨肌肉缩的治疗潜力.
更多相关视频
08:39Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides
Published on: September 16, 2020
09:02Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
Published on: June 9, 2017
相关概念视频
The Electron Transport Chain
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
Mitochondria
Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin
The binding of dantrolene to the RYR1...
Translocation of Proteins into the Mitochondria
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
