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复合水牛 (Bubalus bubalis) 精子生理学上的多细胞体表达,净化和功能特征富含囊蛋白的分泌蛋白1

Nikki Kumari¹, Om Prakash¹, Rajani Kr Paul¹

¹Division of Animal Biochemistry, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India.

International journal of biological macromolecules

|November 8, 2025

概括

在大肠杆菌中产生了复合水牛的氨酸丰富分泌蛋白1 (CRISP-1). 这种生物活性蛋白抑制精子的运动和电容,显示了水牛精液保存的潜力.

科学领域:

生殖生物学生殖生物学
生物化学生物化学
生物技术是生物技术.

背景情况:

富含氨酸的分泌蛋白1 (CRISP-1) 是一种已知能降低精子能力的生长细胞糖蛋白.
防止过早的精子电容对于在精子保存期间延长生育能力至关重要.

研究的目的:

在大肠杆菌中产生生物活性复合水牛CRISP-1的产生.
为了评估其对水牛精子运动,电容和信号通路的影响.

主要方法:

使用大肠杆菌菌株和Ni-NTA亲和染色学优化了水牛CRISP-1 (C15-C242) 的表达和净化.
通过质谱测量证实了蛋白质的身份.
在不同的pH值和温度下评估功能活动.

主要成果:

成功生产和净化 (>90%) 的生物活性重组水牛CRISP-1.
显著降低了精子的渐进性运动和电容,抑制了关键蛋白质氨酸酸化 (p47,p72).
在pH值6.0-9.0 (最佳pH值8.0) 之间表现出活性,热可溶性超过60°C. 通过NO*,AC/cAMP和Ca2+通路抑制HCO3−和L-氨酸诱导的电容.

结论:

重组水牛CRISP-1具有生物活性,有效抑制精子运动和容量.
在水牛精液保存中,CRISP-1显示出有希望的应用,以减轻冷能力.

关键词:

细菌的生产细菌的生产机械特征的表征.再组合水牛CRISP-1蛋白质是什么

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