基于红素的纳米酶作为一种自由基清除剂,用于UV诱导的DNA保护
Lijun Li1, Xue Zhang2, Fan Du2
1Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, 100083, China; China National Pulp and Paper Research Institute Co., Ltd., Beijing, 100102, China.
International journal of biological macromolecules
|November 8, 2025
概括
一种新型的辅助氨基化红素纳米酶 (Zn-AL) 显示出强烈的超氧化物脱酶类活性. 这种可持续的纳米酶有效地清除反应性氧物种,并保护DNA免受氧化损伤.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 绿色化学 绿色化学
背景情况:
- 纳米酶具有类似于酶的催化特性,但具有增强的稳定性.
- 使用自然,环保材料开发可持续的纳米酶对于更广泛的应用至关重要.
- 金属--碳 (M-N-C) 结构对纳米酶活性有希望.
研究的目的:
- 合成一种具有超氧化物脱酶 (SOD) 类活性的可持续配合氨基化木质素纳米酶 (Zn-AL).
- 为了研究纳米酶能够清除反应性氧物种 (ROS) 的能力.
- 评估纳米酶的稳定性,可重复使用性,以及对氧化DNA损伤的保护作用.
主要方法:
- 工业性木质素的环氧氨基化,以产生氨基化木质素.
- 离子与氨基化木质素的协调形成Zn-Nx中心.
- 测试测量超氧化物脱酶 (SOD) 类活性和ROS (超氧化物离子,ABTS基,基) 的清理.
- 在紫外线照射下进行热稳定性测试,可重复使用性实验和DNA保护测试.
主要成果:
- 合成的Zn-AL纳米酶表现出显著的SOD类活性.
- Zn-AL有效地清除了多个ROS,包括超氧化离子,ABTS基和基.
- 该纳米酶表现出优异的热稳定性 (在20-80°C时超过90%的活性) 和可重复使用性 (在5个循环后超过80%的活性).
- 在0.25 mg mL-1的度下,Zn-AL保护了小牛胸腺DNA免受UV诱导的氧化损伤.
结论:
- Zn-AL是一种强大的,可持续的,高效的纳米酶,具有显著的ROS清理能力.
- 这些发现凸显了使用像素这样的改性自然材料来制造先进的纳米酶的潜力.
- 在减轻氧化应激和相关条件方面,Zn-AL显示出有前途的应用.
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