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经过控制剂量后,LC-QTOF通过LC-QTOF识别出二醇代谢物

Svante Vikingsson¹, Ruth E Winecker¹, Katherine Bollinger¹

¹Center for Forensic Science Advancement and Application, RTI International, 3040 East Cornwallis Rd., Research Triangle Park, NC, 27709, USA.

Journal of analytical toxicology

|November 8, 2025

概括

这项研究确定了尿液中的十三种大麻 (CBD) 代谢物,包括新型化合物. 7-基-CBD是水解尿样中的CBD暴露的可靠标志物.

科学领域:

药理学药理学是指药理学的学科.
分析化学分析化学

背景情况:

大麻素 (CBD) 是一种具有治疗潜力的非麻醉性大麻素,在2018年农业法案之后广泛使用.
由于使用量增加,在生物样本中精确测量CBD及其代谢物至关重要.
现有的研究已经确定了一些CBD代谢物,但需要进行全面的分析.

研究的目的:

为了识别和描述人体尿液中的CBD代谢物.
为了确定7-基-CBD和7-碳基-CBD是否是CBD的主要尿路代谢产物.
调查不同CBD配方和施用途径对代谢物概况的影响.

主要方法:

使用液体染色学四极飞行时间质谱法 (LC-QTOF-MS) 对34个控制CBD剂量 (100毫克) 的尿样进行分析.
样品是在不同的时间点 (1.5-22小时) 收集的,代表了不同的配方 (Epidiolex,糖,蒸发).
分析了尿液样本,包括水解和不含水解,以评估代谢物结合.

主要成果:

确定了13种CBD代谢物,包括氧化,碳酸酸形成,基损失和二二醇形成.
最丰富的代谢产物是7-基-CBD,6α-基-CBD,以及一种新的基侧链氧化代谢产物.
超过90%的代谢物是结合的,需要水解来检测;代谢物度在口服后4-6小时达到峰值.

结论:

7-基-CBD是评估CBD在化尿中的暴露的合适标志物.
确定了新的CBD代谢物,提高了对CBD药理动学的理解.
根据配方,CBD度有所不同,Epidiolex与口服糖或蒸发的CBD相比显示出更高的水平.

关键词:

大麻二醇 (Cannabidiol) 是一种在 LC-QTOF 中.代谢代谢代谢代谢药物动力学药物动力学

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