VvLBD11/28抑制了安托氨酸的生物合成和细胞内运输,以减少它们在葡萄中的沉积
Mingxin Feng1, Xuqiao Jin1, Guipeng Liu1
1Shaanxi Key Laboratory for Viti-Viniculture, College of Enology, Northwest A&F University, Yangling 712100, China.
葡萄的侧面器官边界域 (LBD) 蛋白质VvLBD11和VvLBD28抑制了安东素的产生. 它们抑制基因表达并阻断安东素运输,揭示了葡萄中的双重调节机制.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 生物化学 生物化学
背景情况:
- 安西是重要的植物颜料,具有营养益处.
- 侧面器官界域 (LBD) 蛋白质是植物发育中的关键转录因子.
- 对于LBD蛋白在调节氨酸沉积中的确切作用尚不清楚.
研究的目的:
- 为了识别和描述葡萄 (葡萄藤) (Vitis vinifera) LBD转录因子,涉及到安托素积累.
- 阐明这些LBD蛋白调节氨酸生物合成和运输的分子机制.
主要方法:
- 酵母两杂交测定和共同免疫沉以确定蛋白质相互作用.
- 使用定量PCR对基因表达的分析.
- 定位研究以确定核和细胞质中的蛋白质功能.
主要成果:
- 两种葡萄LBD蛋白,VvLBD11和VvLBD28,被确定为反素积累的负调节者.
- VvLBD11/28与TOPLESS联合抑制剂相互作用,并破坏MBW转录复合体,抑制素基因表达.
- 此外,VvLBD11/28还会干扰阳载体VvGST4,从而减少真空运输.
结论:
- VvLBD11和VvLBD28采用双重机制来抑制葡萄中安托素的积累.
- 这涉及同时抑制安托氨酸生物合成和细胞内运输通路.
- 这些发现为转录和转录后调节水果色彩提供了新的见解.
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