一个HDG11介导的转录网络调节了木中的1-deoxynojirimycin生物合成
Zhen Yang1, Shuo Zhao1, Yan Li1
1State Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, China.
Plant physiology
|November 9, 2025
概括
研究人员发现了一种监管网络,控制了木植物中的1-deoxynojirimycin (DNJ) 生产. 这个网络以HDG11转录因子为中心,集成植物激素和发育信号以促进DNJ生物合成.
科学领域:
- 植物分子生物学 植物分子生物学
- 代谢工程是代谢工程.
- 植物激素信号传递
背景情况:
- 1-deoxynojirimycin (DNJ) 是一种药理学上重要的免疫糖,在树 (Morus spp. ) 的情况.
- 墨中DNJ生物合成的精确转录调节在很大程度上仍然没有特征.
- 了解这些机制对于通过代谢工程增强DNJ生产至关重要.
研究的目的:
- 阐明了多层转录网络,控制了木中的DNJ生物合成.
- 确定关键的转录因子及其在调节DNJ生产中的作用.
- 研究植物激素对DNJ生物合成的影响.
主要方法:
- 综合转录基因分析,分子分析和植物激素分析.
- 权重基因共同表达网络分析 (WGCNA) 以确定DNJ相关的基因模块.
- 促进者-报告者测定和蛋白质-蛋白质相互作用研究,以验证监管关系.
主要成果:
- 确定了一个DNJ相关模块 (MEblue),涉及四个转录因子:ZFHD6,HDG11,TCP14和ATHB40.
- ZFHD6,TCP14和ATHB40直接激活了MnGutB1促进体,这是一个关键的DNJ生物合成基因.
- HDG11通过转录激活ZFHD6,TCP14和ATHB40,并形成蛋白质复合体,间接促进DNJ生物合成.
- 植物激素转氨酸核酸和酸诱导HDG11和MnGutB1的表达,增加了DNJ水平.
结论:
- 一个以HDG11为中心的调节层次控制着木中的DNJ生物合成.
- 这个网络集成了植物激素和发育信号,用于DNJ生产.
- 这些发现为植物中DNJ的代谢工程提供了宝贵的见解.
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