在成年和发育中的鼻膜上,opsin 3和opsin 5的表达模式相反
Ramanujam Karthikeyan1,2, Anna-Carin Hägglund1, Ebba Bengtsson1
1Department of Medical and Translational Biology, Umeå University, Umeå, Sweden.
Chemical senses
|November 9, 2025
概括
奥普辛3 (Opn3) 存在于嗅觉感官上皮质中,而奥普辛5 (Opn5) 存在于鼻腔的呼吸上皮质中. 这些opsins可能有助于区分这两种不同的鼻子组织类型.
科学领域:
- 鼻腔生物学 鼻腔生物学
- 感觉和呼吸上皮质分化分化.
- 非视觉的Opsin功能是非视觉的
背景情况:
- 鼻腔含有独特的嗅觉和呼吸上皮质,具有专门的功能.
- 嗅觉感官神经元的分子标记物定义很好,但呼吸上皮细胞的标记物理解较少.
- 在发育的嗅觉区域中检测到非视觉光受体,如opsin 3 (Opn3),这促使人们对它们在鼻上表皮中的作用和存在进行了调查.
研究的目的:
- 研究非视觉光受体,特别是Opn3和Opn5在发育和成人鼻腔中的表达模式和潜在作用.
- 确定Opn3和Opn5是否可以作为分子标记,以区分嗅觉和呼吸上皮质.
- 评估Opn3和Opn5缺乏对鼻上皮质形态和嗅觉功能的功能影响.
主要方法:
- 使用Opn3-eGFP和Opn5-td番茄记者小鼠来跟踪Opsin表达.
- 通过使用像Foxj1,Ker8,OMP,Sox2和Tubb3.3这样的标记物进行了免疫组织化学.
- 分析了成年Opn3淘汰赛 (Opn3-/-) 和Opn5淘汰赛 (Opn5-/-) 小鼠的形态和基因表达模式.
- 通过埋藏食物测试,评估了淘汰小鼠的嗅觉功能.
主要成果:
- 在整个发育过程中,Opn3的表达局限于嗅觉感官领域,而Opn5在晚期的呼吸上皮质上升调节.
- 在成年小鼠中,在感觉上皮的Sox2 / Ker8阳性支柱细胞中发现了Opn3,Opn5留在呼吸上皮中.
- 野生类型和Opn3-/-或Opn5-/-小鼠之间没有观察到形态,基因表达或嗅觉功能的显著差异.
结论:
- Opn3和Opn5在鼻腔中表现出不同的表达模式,Opn3在感觉上皮质中,Opn5在呼吸上皮质中.
- 这些素与Sox2/Ker8标记物一起,有可能区分嗅觉和呼吸上皮质.
- 缺少Opn3或Opn5并没有损害嗅觉功能或改变小鼠鼻上皮质形态,这表明在这些方面有冗余或不必要的作用.
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