在乳牛的周周周期期间,T辅助细胞的代谢和功能变化
T Fernandez-Wallace1, U Arshad1, M Mancheno1
1Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706.
Journal of dairy science
|November 9, 2025
概括
在分娩前,乳牛在产后表现出增强的T辅助细胞代谢和增殖. 这表明奶牛的免疫功能,特别是T辅助细胞,在分娩时不会被抑制.
科学领域:
- 兽医免疫学 兽医免疫学
- 动物的新陈代谢
- 乳牛健康 乳牛健康 乳牛健康
背景情况:
- 周周分娩期显著改变奶牛生理学,增加疾病风险并影响免疫功能.
- 在这个关键时期,T辅助细胞 (CD4+) 的特定作用尚不清楚.
- 了解T辅助细胞的功能对于改善奶牛健康和生产率至关重要.
研究的目的:
- 为了研究乳牛在周周期间的T辅助细胞的体外代谢功能,增殖能力和表型.
- 为了确定分娩后T辅助细胞功能是否被抑制或增强.
- 为了解过渡期奶牛T辅助细胞的免疫状况提供见解.
主要方法:
- 从霍尔斯坦牛中分离出周围血液单核细胞,相对于分娩的时间,相对于分娩的28天,分别是-28,+3和+28天.
- 使用磁分类来净化T辅助细胞.
- 使用海马分析仪评估了新陈代谢功能 (糖解,氧化酸化).
- 在康卡纳瓦林A刺激后,通过细胞痕迹追踪测量了增殖能力.
- 分析了免疫/代谢标记物的细胞因子产生和基因表达.
主要成果:
- 激活的T辅助细胞在+3和+28天表现出糖分和氧化代谢的增加,相对于分娩28天相比.
- 辅助T细胞扩张指数在+3天 (2.65) 最高,其次是+28天 (2.38) 和-28天 (1.91).
- 预炎性细胞因子水平 (IL-1α,IL-1β,IL-6) 在+3和+28天相比-28天升高,没有显著的基因表达变化.
结论:
- 乳牛在分娩后的T辅助细胞代谢和繁殖能力得到了上调.
- 这些细胞在分娩后表现出一种更有利于炎症的表型.
- 在乳牛中,T辅助细胞的免疫功能在周周期间似乎没有被抑制.
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