绿色分析方法开发和验证使用RP-HPLC批量和内部药片剂型的Flavokawain A
Vaishnavi Pawar1, Vaibhav Kadam1, Bhatu Patil1
1R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur District-Dhule, Maharashtra, 425405, India.
Annales pharmaceutiques francaises
|November 9, 2025
概括
一种新的,环保的HPLC方法准确量化了Flavokawain A,一种具有治疗潜力的卡瓦化合物. 这种经过验证的技术确保了在散装药物和药片中的Flavokawain A的可靠质量控制.
科学领域:
- 分析化学 分析化学
- 药理学是指药理学,即药理学是指药理学.
- 绿色化学 绿色化学
背景情况:
- 弗拉沃卡瓦因A是一种来自Piper methysticum (kava-kava) 的石膏,具有显著的抗炎,止痛,心脏保护和抗癌特性.
- 尽管它具有治疗潜力,但缺乏用于量化Flavokawain A的验证分析方法.
研究的目的:
- 开发和验证一种简单,灵敏和环保的反相高性能液态染色法 (RP-HPLC) 方法.
- 为了能够准确量化大量药物和药片配方中的Flavokawain A.
主要方法:
- 使用Shim-pack GIST C18列进行RP-HPLC分离.
- 甲醇:水 (85:15 v/v) 移动阶段,流量为1.0 mL/min.
- 方法验证包括线性,灵敏度 (LOD/LOQ),准确度和精度.
主要成果:
- 弗拉沃卡温A在4.8分钟内化,在2-12微克/毫升的范围内具有很好的线性 (R2=0.9999) .
- 获得高灵敏度的LOD为0.281μg/mL,LOQ为0.853μg/mL.
- 准确和精确的量化与恢复 (99.2-101.3%) 和低%RSD (<2%);测定结果显示了100.1%的散装和99.5-101.7%的片.
结论:
- 开发的RP-HPLC方法简单,准确,精确,并且对环境可持续 (AGREE评分为0.79).
- 这种经过验证的方法适用于药物配方中的常规质量控制和基于研究的Flavokawain A量化.
关键词:
分析制药公司的药品.这是一种环保的方法.弗拉沃卡韦恩是A队的队长弗拉沃卡韦因A是一种绿色的RP-HPLC可以使用.方法验证方法的验证方法.生态负责任的方法制药分析 制药分析Piper methysticum 是一家在西班牙的公司.在RP-HPLC中,我们可以看到验证方法的验证.更多相关视频
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