通过客宿主相互作用对颗粒状水凝的动态调节,以时空地指导细胞迁移
Keisuke Nakamura1, Nikolas Di Caprio1, Jonathan T Taasan2
1BioFrontiers Institute, University of Colorado Boulder, Boulder, CO, 80303, USA.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|November 10, 2025
概括
化学反应性水凝可以动态控制3D细胞迁移. 该平台可为先进的组织工程应用实现细胞运动的精确,按需调制.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
背景情况:
- 细胞迁移对于组织发育,再生和修复至关重要.
- 在3D空间和时间中控制细胞迁移是当前细胞培养平台面临的挑战.
研究的目的:
- 开发一种新的平台,使用化学响应的颗粒状水凝来动态控制3D细胞迁移.
- 为了研究基于氨酸的微凝的使用,具有可逆的客宿主相互作用,以调节材料特性和细胞行为.
主要方法:
- 动态微凝的制造,使用与阿达曼坦和β-环氧德克斯特林交联的氨酸.
- 加入阿达曼坦碳酸 (Ad-COOH) 来诱导胀并改变水凝的特性 (硬度,孔径大小).
- 生物打印中酶体 stromal 细胞 (MSC) 球体变成动态颗粒状水凝,以创建具有图案细胞区域的 4D 结构.
主要成果:
- 添加Ad-COOH降低了微凝的刚度,增加了孔径,调节了3D微环境.
- 在添加Ad-COOH时,MSC从球体的迁移减少,在去除后恢复,表明时间控制.
- 4D打印的结构展示了有图案的细胞区域,具有对细胞退出的时空控制,创造了复杂的细胞安排.
结论:
- 化学响应的颗粒状水凝提供精确的,按需调制3D细胞迁移.
- 这个平台可以制造具有控制的细胞模式的动态,复杂的工程组织.
- 该战略为先进的组织工程和再生医学应用提供了新的机会.
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