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草甲状腺酶抑制干扰素调节因子-7促进草甲状腺复原病毒的复制

Jinglong Wang¹, Lu Yan¹, Junru Wang²

¹College of Water Resource and Modern Agriculture, Nanyang Normal University, Nanyang, China.

Journal of fish diseases

|November 10, 2025

概括

草重生病毒 (GCRV) 感染增加了氧化应激. 草触酶 (CiCat) 酶通过减少CiIRF-7表达来促进GCRV复制,从而阻碍抗病毒反应.

科学领域:

水产养殖是水产养殖的一种方式.
鱼类免疫学鱼类免疫学
分子生物学分子生物学

背景情况:

草鱼 (Ctenopharyngodon idellus) 水产养殖受到草鱼重生病毒 (GCRV) 的威胁.
GCRV感染通过反应性氧物种 (ROS) 的过度生产,导致鱼类细胞的氧化应激.
catalase (Cat) 是一种抗氧化酶,可以中和过氧化 (H2O2).

研究的目的:

调查CiCat在草对GCRV感染的反应中的作用.
在GCRV挑战期间描述CiCat表达,定位和功能.

主要方法:

在各种组织中对CiCat的定量表达分析.
光显微镜以确定CiCat细胞局部化和与GCRV的同局部化.
细胞测试以评估CiCat过度表达对ROS水平和GCRV复制的影响.
对CiCat与干扰素调节因子-7 (IRF-7) 的相互作用进行分析.

主要成果:

在所有测试的草鱼组织中都表达了CiCat,并在GCRV挑战时显著上调.
GCRV感染增强了CiCat的聚合和与线粒体和内分泌网膜的同定位.
过度表达CiCat可以降低ROS,但矛盾的是,它促进了GCRV的复制.
通过减少CiIRF-7表达,CiCat抑制了抗病毒反应.

结论:

在草中,CiCat扮演着双重的角色,缓解氧化应激,同时促进GCRV复制.
CiCat通过抑制宿主的干扰素调节因子-7 (IRF-7) 抗病毒途径来增强GCRV复制.
针对CiCat活动可能是GCRV疾病管理在草养殖中的潜在策略.

关键词:

催化酶的催化剂是什么草地鱼的reovirus是一种病毒.干扰素调节因子-7-7

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