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通过水溶性拉曼传感器可视化细胞中的硫醇应激反应

Hiroyuki Yamakoshi¹, Meichen Wang¹, Keisuke Koga¹

¹Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan.

Analytical chemistry

|November 10, 2025

概括

精确的活细胞醇量化现在可以通过iPrCAA,最小的比度拉曼探针. 这种新型的探测器能够对细胞内醇水平和分布进行敏感的实时监测.

科学领域:

化学生物学化学生物学
分子成像学分子成像学
细胞生物学细胞生物学

背景情况:

由于光探针的局限性,难以准确量化活细胞中的细胞内醇.
现有的探测器受到信号和,光漂白和染料聚合的影响,阻碍了可靠的测量.

研究的目的:

引入 (E) -2-cyano-3-isopropylacrylamide (iPrCAA) 作为一种用于量化和可视化细胞内硫醇度的新型比度拉曼探针.
为了证明iPrCAA对实时监测活细胞中醇动态的实用性.

主要方法:

开发和描述iPrCAA,这是用于活细胞分析的最小的醇检测探头.
使用iPrCAA作为一个比度拉曼探针,用于敏感的醇检测.
在没有硫醇的介质中监测细胞内硫醇的耗尽,并可视化硫醇的分布.

主要成果:

iPrCAA是现有的最小的醇检测探头 (138 g/mol),具有高反应性和水溶性,提高了灵敏度.
实时监测显示,在没有醇的介质中,醇水平降低了约3倍.
可视化醇分布与细胞内的蛋白质分布密切相关.

结论:

iPrCAA提供了一种实用而敏感的方法,用于量化活细胞中的内源性硫醇.
这种拉曼探测器能够动态跟踪醇波动,特别是在细胞应激条件下.
iPrCAA克服了光探针的局限性,用于准确的细胞内硫醇测量.

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