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相关概念视频

Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

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ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
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Transcytosis of IgG01:15

Transcytosis of IgG

3.4K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
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Western Blotting01:15

Western Blotting

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Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Protein Denaturation01:28

Protein Denaturation

8.8K
The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
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Bacterial Protein Maturation01:26

Bacterial Protein Maturation

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Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
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相关实验视频

Updated: May 3, 2026

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
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Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

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在中风后,微质显示改变的空间形态和蛋白质组.

Brooke J Wanrooy1, Jenny L Wilson1, Althea R Suthya1

  • 1Centre For Inflammatory Diseases, Department of Medicine, School of Clinical Sciences At Monash Health, Monash University, Victoria, Clayton, Australia.

Proteomics
|November 10, 2025
PubMed
概括

缺血性中风会改变大脑的微环境,显著影响微质形态和功能. 这些免疫细胞显示出特定区域的变化,影响中风恢复至关重要的炎症信号通路.

关键词:
微质细胞中的微质细胞形态学 形态学 形态学神经炎症是一种神经炎症.蛋白质组是蛋白质组的组成部分.一次性中风中风中风中风中风

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A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology
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A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology

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Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
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Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

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相关实验视频

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A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology
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科学领域:

  • 神经科学是一个神经科学.
  • 免疫学 免疫学 免疫学
  • 脑卒中研究 脑卒中研究

背景情况:

  • 微质细胞是中枢神经系统 (CNS) 中的关键免疫细胞,对神经发育和恒常状态至关重要.
  • 它们充当免疫哨兵,不断监控神经环境,并对大脑的侮辱作出反应.

研究的目的:

  • 研究由缺血性中风改变的大脑微环境如何影响微质形态和功能以空间依赖的方式.
  • 为了确定中风后微质中的分子变化.

主要方法:

  • 动物中的过渡性中脑动脉封闭 (tMCAO) 模型.
  • 通过3D重建分析区域基因表达,微质形态,以及中风后24小时的公正蛋白质组学.
  • 微质细胞与半球的异侧和异侧分离.

主要成果:

  • 与虚假对照相比,心脏病核心表现出明显的促炎微环境.
  • 脑卒中会诱导特定区域的微质形态变化,具有亚米形状和心脏病发作附近的复杂性降低.
  • 在微质中发现了108种差异表达的蛋白质,涉及细胞因子生产,化学因子活性和白细胞迁移的途径.

结论:

  • 微细胞的形态和功能因缺血性中风而显著改变,以特定区域的方式.
  • 这些变化与独特的炎症特征和大脑内的蛋白质表达变化有关.
  • 微质细胞在调节中风后的炎症信号传递中起着至关重要的作用.