相关实验视频

Updated: Jan 11, 2026

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

心脏纤维细胞衍生的IGFBP6通过合EGR1-MFAP4轴来协调心脏重塑

Shaopeng Cheng^1,2, Yilin Wang^3,2, Kunsheng Li^1,2

¹Department of Cardiothoracic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu, China.

International journal of biological sciences

|November 10, 2025

概括

纤维细胞衍生的胰岛素类生长因子结合蛋白6 (IGFBP6) 通过促进肌纤维细胞分化来驱动心脏纤维化. 向IGFBP6-EGR1-MFAP4通路可以治疗心脏重塑障碍.

科学领域:

心血管生物学心血管生物学
纤维化研究纤维化研究
分子医学是分子医学.

背景情况:

纤维细胞向肌纤维细胞过渡 (FMT) 驱动心脏纤维化和不良重塑.
胰岛素类生长因子结合蛋白6 (IGFBP6) 调节细胞生长,并与纤维化反应有关.
IGFBP6在心脏纤维细胞激活和重塑中的特定作用尚未完全理解.

研究的目的:

研究IGFBP6在纤维细胞介导心脏重塑中的作用.
阐明IGFBP6调节肌纤维细胞分化的分子机制.
评估针对心脏纤维化中的IGFBP6通路的治疗潜力.

主要方法:

在心脏纤维化数据集和患者血清中分析IGFBP6表达.
产生的心脏纤维细胞和肌纤维细胞特异性IGFBP6淘汰赛小鼠.
评估心脏功能和心肌梗塞 (MI) 或异二醇 (ISO) 挑战后的重塑.
利用LC-MS/MS和RNA测序来发现IGFBP6-介导的FMT的分子机制.

主要成果:

在纤维性心脏中,IGFBP6的表达上调,与慢性心脏病相关.
IGFBP6淘汰赛减弱后MI和ISO诱导的心脏重塑和功能障碍.
抑制IGFBP6抑制了TGF-β1诱导的FMT.
IGFBP6与EGR1相互作用,增强EGR1与MFAP4促进体的结合,这是一个亲纤维细胞介质.

结论:

来自纤维细胞的IGFBP6是一种通过EGR1-MFAP4轴的心脏纤维化新型调节剂.
IGFBP6促进肌纤维细胞分化和不良的心脏重塑.
准IGFBP6通路为心脏重塑障碍提供了潜在的治疗策略.

关键词:

在 EGR1 里面,你会看到 EGR1 的位置.在IGFBP6中使用.在MFAP4中,MFAP4是MFAP4.的心脏纤维化.纤维细胞细胞是什么?肌纤维母细胞细胞.

更多相关视频

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...