蛋白纳米双体的配方和表征,以提高吸收能力
Saisree Iyer1, Mamatha B Sheshappa1
1Department of Food Safety and Nutrition, Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Paneer Campus, Deralakatte, Mangaluru, Karnataka 575018 India.
Journal of food science and technology
|November 10, 2025
概括
研究人员开发了蛋白纳米双胞体 (LNB),以提高蛋白的生物可用性. 这些LNB显示改善了胃肠道稳定性和肠道透性,为眼睛和大脑健康补充剂提供了一个有前途的输送系统.
科学领域:
- 营养学科学 营养学科学
- 纳米技术 纳米技术
- 生物化学 生化学
背景情况:
- 氨酸是一种托菲尔类胡卜素,具有显著的眼睛和大脑健康益处.
- 它的治疗潜力受限于由于疏水性和低胃肠道稳定性而导致的生物可用性差.
研究的目的:
- 开发和表征蛋白纳米双体 (LNB) 以提高蛋白的生物可用性.
- 为了评估LNB的体外胃肠道稳定性和肠道透.
主要方法:
- 使用动态光散射 (DLS),传输电子显微镜 (TEM) 和里埃转换红外光谱 (FT-IR) 准备和表征了氨酸纳米双体 (LNB).
- 在体外模拟的消化模型中评估了胃肠道稳定性和肠道透性.
- 捕获效率 (%EE) 和存储稳定性也得到了评估.
主要成果:
- LNB 呈现出 117.9 nm 的 zeta 尺寸, 0.246 的多分散度指数和 -65 mV 的 zeta 潜力.
- TEM证实了球形LNB的尺寸在20-50nm之间.
- 观察到高捕获效率 (>90%) 和出色的存储稳定性 (>8周).
- FT-IR分析表明,在LNB内没有黄蛋白降解.
- 与自由黄蛋白相比,LNB显示了5倍以上的胃肠稳定性和大约70%的肠道透率.
结论:
- 氨酸纳米双体体 (LNB) 是有效的载体,可以改善氨酸的生物可用性.
- LNB显示出在补充剂和营养保健品中用于管理与氧化压力相关的疾病的潜力.
- 这种纳米配方可以帮助预防与年龄相关的黄斑变性 (AMD),糖尿病视网膜病变和白内障等疾病.
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