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呼吸活检生物标志物:呼出的呼吸凝聚物中的无细胞核酸

Ondrej Pös^1,2, Jakub Styk^1,2, Monika Kubanova^1,3

¹Comenius University Science Park, Bratislava, Slovakia.

Frontiers in medicine

|November 10, 2025

概括

对无细胞核酸 (cfNAs) 的呼气凝聚物 (EBC) 分析提供了一种非侵入性的方法来诊断呼吸系统疾病. 目前正在进行的研究旨在克服更广泛的临床应用的挑战,并可能补充血液测试.

科学领域:

生物分子分析.
呼吸系统药物呼吸系统药物
非侵入性诊断是一种非侵入性诊断.

背景情况:

呼气凝结物 (EBC) 是一种有前途的生物流体,用于非侵入性诊断.
最近的重点已经从挥发性有机化合物转移到EBC中的非挥发性无细胞核酸 (cfNA).
在EBC中cfNA可以反映呼吸道生物学,并作为生物标志物.

研究的目的:

审查EBC中cfNAs的当前知识.
突出cfNA分析的技术挑战和优化策略.
检查EBCcfNA分析的新兴应用.

主要方法:

对EBC中cfNAs的现有文献的审查.
比较EBC采集装置和核酸提取技术.
分析诊断和疾病监测中的新兴应用.

主要成果:

在EBC中的cfNA包括DNA,RNA和微生物遗传物质,反映了呼吸系统健康状况.
采样和提取过程中的技术挑战影响了cfNA的产量和标准化.
新兴应用显示了早期癌症检测,感染诊断和疾病内型鉴定的潜力.

结论:

在非侵入性诊断方面,EBC cfNA 分析具有显著的翻译潜力.
需要进一步创新采样和分子技术,以解决局限性问题.
脑电细胞分析可以补充基于血液的液体活检,提供器官特定的见解.

关键词:

呼吸活检活检没有细胞的核酸.呼出的呼吸凝凝液 (EBC) 是一种液体活检活检液体活检肺癌生物标志物肺癌生物标志物阻塞性肺部疾病阻塞性肺部疾病

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