活力差异化增强了微生物群落在商业准备食用肉制造业中的概况
Jessica A Brown1, Steven C Ricke1
1Meat Science and Animal Biologicals Discovery (MSABD) Program, Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, United States.
Journal of applied microbiology
|November 10, 2025
概括
16S rRNA基因测序与一氧化 (PMA) 处理增强了准备食用 (RTE) 肉制造中的微生物污染检测. 这种方法可以更好地识别可行的腐烂污染物.
科学领域:
- 食品微生物学 食品微生物学
- 分子诊断学 分子诊断学
- 这是下一代测序.
背景情况:
- 准备食用 (RTE) 肉中的微生物污染构成了食品安全风险.
- 准确检测可活性的污染物对于RTE肉类生产的质量控制至关重要.
研究的目的:
- 评估16S rRNA基因测序与生命能力差异化相结合,用于检测RTE肉中的微生物污染.
- 为了评估单 (PMA) 治疗在改善序列分辨率方面的疗效.
主要方法:
- 在各种制造阶段 (原料,后,冷却,持有,分阶段,切片,包装) 采集的RTE肉样.
- 应用胺 (PMA) 处理,以区分可活和不可活的微生物细胞.
- 提取了DNA,进行了16S rRNA基因测序,并进行了生物信息分析.
主要成果:
- PMA治疗减少了微生物群落的丰富性,但在样本位置的影响有所不同.
- 在对照样本和PMA处理样本中,热处理对微生物群落的影响相似.
- 切片诱导了PMA处理的样本中的二次微生物群落转移,增加了Lactobacillus丰富度,通过培养证实了这一点.
结论:
- 甲酸 (PMA) 治疗显著提高了16SrRNA基因测序的分辨率.
- 这种改进的分辨率有助于在RTE肉制造过程中精确识别可行的腐烂微生物.
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