Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches01:23

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches

392
Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
392
Recombinant DNA01:09

Recombinant DNA

101.5K
Overview
101.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Network Formation Dynamics in Thiol-ene Crosslinked Hyaluronic Acid Hydrogels: Design Principles for In Vitro Tissue Models.

bioRxiv : the preprint server for biology·2026
Same author

Enzymatic Methods for Assembling and Modifying Hydrogel Biomaterials.

Regenerative engineering and translational medicine·2026
Same author

A robust and user-agnostic step-emulsion platform for scalable microgel fabrication.

bioRxiv : the preprint server for biology·2026
Same author

De novo design of protein nanoparticles with integrated functional motifs.

bioRxiv : the preprint server for biology·2026
Same author

Suspended Tissue Engineering with Assemblable Microfluidics (STEAM).

bioRxiv : the preprint server for biology·2025
Same author

From sequence to scaffold: Computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocks.

Proceedings of the National Academy of Sciences of the United States of America·2025

相关实验视频

Updated: Jan 11, 2026

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.5K

基于布尔逻辑的生物活性蛋白的控制释放,具有多样化的输入.

Murial L Ross1, Ryan Gharios2, Shivani Kottantharayil1

  • 1Department of Bioengineering, University of Washington, Seattle, WA, 98105, USA.

Angewandte Chemie (International ed. in English)
|November 10, 2025
PubMed
概括

研究人员开发了一种使用蛋白质组装来控制生物材料中各种生物活性蛋白质的释放的新方法,该方法基于特定的蛋白酶组合或光信号. 这促进了针对药物输送等应用程序的基于逻辑的控制.

关键词:
生物活性治疗药物布尔逻辑是一个布尔逻辑.药物交付是药物交付的过程.蛋白质工程是一种蛋白质工程.刺激-响应性的刺激.

更多相关视频

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
10:07

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

1.7K
Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
08:58

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

572

相关实验视频

Last Updated: Jan 11, 2026

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.5K
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
10:07

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

1.7K
Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
08:58

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

572

科学领域:

  • 生物材料科学 生物材料科学
  • 分子工程分子工程分子工程
  • 合成生物学 合成生物学

背景情况:

  • 响应刺激的生物材料对于受控的治疗输送,组织工程和生物传感至关重要.
  • 之前的工作使用基于布尔逻辑的自主编译来从生物材料中释放蛋白质,但仅限于简单的输出和输入.
  • 扩大这一框架对于生物医学中的更广泛应用至关重要.

研究的目的:

  • 扩大基于逻辑的生物材料蛋白质释放的能力.
  • 通过使用3输入运算符集来演示所有七个独特逻辑操作的组合.
  • 为了使各种生物活性蛋白质在对特定刺激的反应中释放出来.

主要方法:

  • 组装了所有7个独特的逻辑操作 (YES/OR/AND) 来控制蛋白质释放.
  • 使用了三个直角蛋白酶执行器来触发蛋白质货物的条件释放.
  • 包含一个可光分解的蛋白质图案,使可见光作为额外的释放输入.

主要成果:

  • 成功证明了各种生物活性蛋白的可编程释放,包括生长因子,酶,纳米体,细胞因子和光蛋白.
  • 从材料中实现基于逻辑的释放,以响应精确的蛋白酶输入组合,保持蛋白质生物活性.
  • 展示了可见光作为基于逻辑的蛋白质释放的可控输入.

结论:

  • 开发的框架显著扩大了基于逻辑的生物材料蛋白质释放的适用性.
  • 这种方法可以精确控制各种功能性生物大分子的释放.
  • 这些发现为在向治疗,诊断和合成生物学等领域的先进应用铺平了道路.