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Updated: Jan 11, 2026

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用质谱图像测量对形式固定,嵌样本中的生物分子进行序列检测

Erin H Seeley¹, Danielle L Stolley², Anna K Casasent²

¹Department of Chemistry, University of Texas at Austin; EHSeeley@mdanderson.org.

Journal of visualized experiments : JoVE

|November 10, 2025

概括

这项研究引入了一种质谱成像 (MSI) 协议,用于分析组织中的代谢物,N链 glycans 和. 这种方法保留了空间信息,使我们能够全面了解复杂的细胞环境中的分子相互作用.

科学领域:

生物医学科学生物医学科学
分析化学分析化学
分子生物学分子生物学

背景情况:

传统的组织分析破坏了空间信息,阻碍了对分子相互作用的研究.
了解细胞内和细胞外分子相互作用对于器官功能至关重要.
质谱成像 (MSI) 通过保存空间数据提供了一个解决方案.

研究的目的:

提出一个从单个组织切片中对代谢物,N链 glycans 和 triptic 进行序列式质谱成像 (MSI) 的协议.
为了使多个生物分子类别的近单细胞分辨率分析.
为了促进MSI数据与其他空间奥米克数据集的集成.

主要方法:

序列质谱成像 (MSI) 在相同的组织部分.
在20μm分辨率下对代谢物,N链 glycans 和 tryptic 进行分析.
仔细处理组织和成像,以保持切片的完整性.

主要成果:

从单个组织部分获得了代谢物,N链 glycans 和 tryptic 的高质量空间图像.
该协议允许检测具有保存空间信息的多个分析物类.
证明了将MSI数据与来自串行段的其他空间空间数据集成的可行性.

结论:

序列MSI提供了组织的全面的空间分子地图.
该协议增强了对细胞环境中的复杂分子相互作用的理解.
能够进行多omics空间分析,以获得更深入的生物学见解.

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