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一个新的UPLC-MS/MS方法来确定Tegoprazan在老鼠血中:在老鼠药理动力学研究中的应用

Mengjie Xu¹, Yize Cai², Yu Wang³

¹Precision Medicine Center, Wenzhou People's Hospital, Wenzhou, China.

Biomedical chromatography : BMC

|November 10, 2025

概括

一种新的UPLC-MS/MS方法可以准确地测量大鼠血中的泰戈普拉桑 (TEG). 药物动力学研究表明,TEG被快速吸收,迅速达到度峰值,有助于胃食道逆流症治疗研究.

科学领域:

药理学药理学是指药理学的学科.
分析化学分析化学

背景情况:

泰戈普拉桑 (TEG) 是胃食道逆流症的关键药物.
在生物矩阵中精确量化TEG对于药理动力学研究至关重要.

研究的目的:

开发和验证一种UPLC-MS/MS方法,用于在老鼠血中量化TEG.
将验证的方法应用于大鼠的药理动力学研究.

主要方法:

超高性能液体染色学-并联质谱学 (UPLC-MS/MS) 具有电喷离子化 (ESI) 阳性模式.
使用revaprazan (REV) 作为内部标准在具有梯度化的Waters ACQUITY UPLC BEH柱上进行分析.
方法验证包括线性,准确性,精度,回收,矩阵效应和稀释完整性.

主要成果:

该UPLC-MS/MS方法表现出极好的线性 (2-1000 ng/mL),精度 (-8.5%至12.2%) 和精度.
观察到高IS-正常化恢复 (109.3%-113.6%) 和最小的矩阵效应 (99.0%-102.8%).
鼠类的药理动力学参数显示TEG吸收迅速,Tmax为1.8 ± 1.1小时,Cmax为2513.2 ± 707.0 ng/mL.

结论:

成功建立了一种快速,稳健和经过验证的UPLC-MS/MS方法,用于在老鼠血中量化TEG.
药物动力学特征表明,TEG在老鼠中被迅速吸收,并达到峰值度.
这种方法有助于进一步研究TEG的治疗应用.

关键词:

在 UPLC MS/MS/MS 中.药物动力学药物动力学鼠标鼠标鼠标鼠标鼠标这就是Tegoprazan的原因.

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