在发酵过程中,Lactobacillus exopolysaccharide和大豆蛋白之间的相互作用:聚合特征和结合动力学
Wanrong Jiang1, Huaiyu Xu1, Xiaoyu Yang1
1College of Food Science, Northeast Agricultural University, Harbin, 150030, China.
International journal of biological macromolecules
|November 10, 2025
概括
来自Lactobacillus casei的外聚糖 (EPS) 在发酵过程中调节大豆蛋白分离物 (SPI) 相互作用. 这项研究阐明了EPS如何影响蛋白质聚合和结合动力学,有助于开发先进的发酵大豆产品.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 微生物学 微生物学
背景情况:
- 乳杆菌casei外聚糖 (CEPS) 在发酵食品中至关重要.
- 大豆蛋白分离物 (SPI) 是许多食品中的关键成分.
- 了解CEPS-SPI相互作用对于优化发酵过程至关重要.
研究的目的:
- 阐明在发酵过程中控制CEPS-SPI相互作用的分子机制.
- 分析CEPS-SPI复合材料的聚合特性和结合动力学.
- 研究CEPS度和pH值对这些相互作用的影响.
主要方法:
- 构建化CEPS-SPI复合系统.
- 聚合特征的分析.
- 研究结合动力学和泽塔潜力.
- 在酸化过程中动力力分析 (pH 7.0-4.5).
主要成果:
- CEPS度和pH值显著调节了CEPS-SPI相互作用.
- CEPS改变了可溶性复合物形成的关键pH点 (pHφ1和pHmax).
- 最低溶解度和零泽塔电位转移到较低的pH值.
- 在酸化过程中确定了三种不同的相互作用力阶段:范德瓦尔斯/键,疏水/静电,静电相互作用.
结论:
- 在发酵过程中,CEPS在调节SPI的功能性质方面发挥着重要作用.
- 这项研究为发酵中的EPS蛋白相互作用提供了理论框架.
- 这些发现支持开发高质量的发酵大豆产品.
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