通过整合代谢工程和适应性实验室进化,在Yarrowia lipolytica中推进基于阿拉比诺斯的生物生产
Razieh Rafieenia1, Jing Fu1, Piotr Hapeta2
1Department of Bioengineering, Imperial College London, London, SW7 2AZ, UK; Imperial College Centre for Engineering Biology, Imperial College London, London, UK; The Microbial Food Hub, Imperial College London, London, UK; Bezos Centre for Sustainable Protein, Imperial College London, London SW7 2AZ, UK.
Metabolic engineering
|November 10, 2025
概括
这项研究设计了Yarrowia lipolytica以利用L-arabinose,一种纤维素素素成分,用于生物生产. 工程酵母成功地从L-阿拉比诺酸中产生β-胡卜素,为可持续制造开辟了新的途径.
科学领域:
- 生物技术和合成生物学
- 微生物工程 微生物工程
- 工业微生物学 工业微生物学
背景情况:
- 雅罗维亚 (Yarrowia lipolytica) 是一种生产有价值化合物的多功能酵母.
- 纤维素是一种丰富的,可持续的生物生产原料.
- Y. lipolytica 不能自然利用所有的纤维素素素成分,包括L-arabinose.
研究的目的:
- 为了设计Yarrowia lipolytica以以L-arabinose作为唯一的碳来源来生长.
- 通过适应性实验室进化来提高L-氨酸的利用率.
- 为了使工程Y. lipolytica. 在工程Y. lipolytica.中从L-arabinose中产生β-胡卜素.
主要方法:
- 对Y. lipolytica进行基因工程,以表达一种L-arabinose利用基因盒.
- 适应性实验室进化以增强L-阿拉比诺斯的同化.
- 在进化的菌株中引入β-胡卜素生物合成基因盒.
主要成果:
- 工程化Y. lipolytica菌株在L-arabinose上表现出增长.
- 适应性进化显著提高了L-阿拉比诺斯利用效率.
- 高水平的β-胡卜素产量 (418.89 mg/l) 由60 g/l的L-阿拉比诺获得.
结论:
- 这项研究为Yarrowia lipolytica中L-阿拉比诺斯利用的首次成功工程展示.
- 开发的菌株为生物制造提供了一个新的平台,使用来自林氏纤维素生物质的L-氨酸.
- 这项研究扩大了Y. lipolytica作为可持续化学品和生物燃料生产的细胞工厂的潜力.
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