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在大视野中提高灵敏度多光谱激光扫描光声显微镜用于在体内测量氧和度

Mohsin Zafar¹, Amir Khansari¹, Rayyan Manwar¹

¹The Richard and Loan Hill Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, Illinois, USA.

Journal of biophotonics

|November 10, 2025

概括

这项研究引入了一种广场多谱激光扫描光声显微镜 (LS-PAM) 系统,用于改善组织中氧和 (sO2) 的测量. 新的波长分组提高了信号与噪声的比率,以获得更准确的结果.

科学领域:

生物医学光学生物医学光学
摄影声学成像技术的成像
组织光谱学组织光谱学

背景情况:

带有刺激拉曼散射 (SRS) 的多谱光声学显微镜 (PAM) 测量组织氧和度 (sO2).
激光扫描光声显微镜 (LS-PAM) 由于传感器的设计和对齐而面临检测灵敏度的限制.
现有的广视场LS-PAM损害了横向分辨率和灵敏度.

研究的目的:

开发一个广泛的多光谱LS-PAM系统,用于增强生物组织中的sO2测量.
改进信号与噪声比 (SNR) 以更准确的sO2量化.
为了克服传统LS-PAM系统的灵敏度限制.

主要方法:

实施一个广泛的多光谱LS-PAM系统.
使用两个波长组:一个异基组 (532 nm和545 nm) 和一个脱氧血球蛋白主导组 (545 nm和558 nm).
验证使用幻影和体内研究.

主要成果:

新的波长分组策略显著改善了检测到的光声信号的SNR.
该系统在测量生物组织中的sO2时表现出更高的准确性.
在受控幻体实验和活生物样本中成功验证.

结论:

开发的广场多谱LS-PAM系统为准确的体内sO2测量提供了有前途的方法.
使用特定波长组有效地提高了检测灵敏度和量化准确度.
这项技术有可能推进研究和临床应用,需要精确的组织氧化监测.

关键词:

在活体中,活体中显微镜显微镜是一种微观的仪器.氧气和和氧气和的情况.摄影声学摄影声学这是光谱学.

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