对H5N1高度致病性禽流感病毒的不同传输媒介的比较评估
Baleshwari Dixit1,2, Harshad Vinayakrao Murugkar3, Shanmugasundaram Nagarajan3
1College of Veterinary Science & A. H., Rewa, Nanaji Deshmukh Veterinary Science University, Jabalpur, MP, India. baleshwarivpe@gmail.com.
Scientific reports
|November 10, 2025
概括
在模拟现场条件下,正常盐水 (NS),酸盐缓冲盐水 (PBS) 和木炭基病毒传输介质 (CVTM) 有效地保持了H5N1流感病毒感染力. 这些经济和可访问的媒介适合在发展中国家具有挑战性的样品运输.
科学领域:
- 病毒学 病毒学
- 微生物学 微生物学
- 公共卫生 公共卫生
背景情况:
- 采样,储存和运输在发展中国家具有挑战性,原因是环境条件不利,运输媒介有限,冷链维护有限.
- H5N1流感病毒对公众健康构成重大威胁,需要可靠的检测和监测方法,特别是在资源有限的环境中.
研究的目的:
- 为了比较五种不同的传输介质 (酸盐缓冲盐水,50%甘+PBS,正常盐水,病毒传输介质和木炭基病毒传输介质) 在模拟现场条件下保持H5N1流感病毒传染性的有效性.
- 确定适合在资源有限的环境中进行样本运输的具有成本效益和易于获得的媒介.
主要方法:
- 五种传输介质 (NS,PBS,50%糖+PBS,VTM,CVTM) 被测试了它们保护H5N1流感病毒感染的能力.
- 含有病毒的便样本和亚兰体液在模拟的现场条件下存储和运输长达7天.
- 病毒隔离每12小时进行一次,使用胚胎蛋注射,然后进行血化 (HA) 测试和RT-PCR进行鉴定.
- 病毒存活率通过计算百分比感染率来量化,并使用后勤回归来分析.
主要成果:
- 正常盐水 (NS),酸盐缓冲盐水 (PBS) 和木炭基病毒传输介质 (CVTM) 在保持H5N1病毒完整性方面表现出最高的有效性.
- 这三个介质很容易获得,经济,准备不那么复杂,使它们适合现场使用.
- 病毒存活率的显著差异仅在艾伦液和便样本之间的病毒输送介质 (VTM) 中观察到.
结论:
- 推NS,PBS和CVTM作为H5N1流感病毒监测的有效和实用的传输媒介,在具有挑战性的现场条件下进行监测,特别是在发展中国家.
- 这些发现支持使用易于获得和经济的媒体来克服用于传染病监测样本运输的后勤障碍.
- 进一步的研究可能会探索这些媒介对其他病毒的长期稳定性和更广泛的适用性.
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