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使用纳米MIPs-aptamer三明治试验的敏感和选择性电化学检测癌胚抗原

Chutimon Akkapinyo¹, Yingyot Poo-Arporn², Ramida Rattanakam³

¹Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok, 10140, Thailand.

Scientific reports

|November 10, 2025

概括

使用分子印记聚合物纳米粒子 (nanoMIPs) 的新型电化学传感器提供了高度敏感的癌胚抗原 (CEA) 检测. 这一进步有助于提高癌症诊断和监测的准确性和成本效益.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
纳米技术纳米技术

背景情况:

癌胚抗原 (CEA) 是癌症诊断,治疗监测和复发检测的关键生物标志物.
现有的CEA检测方法可能缺乏广泛临床应用所需的灵敏度,选择性或成本效益.

研究的目的:

开发一种高度敏感和选择性的电化学传感器,用于癌胚抗原 (CEA) 检测.
利用分子印记聚合物纳米粒子 (nanoMIPs) 和金属有机框架来增强信号传导.

主要方法:

在印碳电极上合成和固定CEA特定的纳米MIP.
使用UiO-66-NH₂金属有机框架进行电化学信号探针的构建,用于离子 (Pb²⁺) 吸附和体功能化.
开发一个纳米MIPs-aptamer三明治试验加上方波阳极剥离电压测量用于CEA量化.

主要成果:

开发的传感器显示出CEA的优秀线性检测范围,从1到1,000 ng/mL.
实现了低检测极限 (1.4 ng/mL),低于CEA的临床切断值.
传感器在增值的人类血清样本中显示出高灵敏度,良好的可重复性,优秀的选择性和令人满意的恢复率 (98.12103.24%).

结论:

基于nanoMIPs-aptamer的电化学传感器为CEA检测提供了一个灵敏,选择性和成本效益的平台.
传感器在人血清样本中的性能突显了其在实际临床诊断应用中的潜力.
这种方法为改善癌症生物标志物监测和管理提供了一个有希望的工具.

关键词:

癌症癌症癌症癌症癌胚抗原是一种癌胚抗原.金属有机框架金属有机框架有分子印记的聚合物.纳米MIPs可以使用.

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