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截断的APC通过准结直肠癌中线粒体上的MAVS来破坏先天免疫反应

Si-Yu Li^1,2, Xin-Yi Wang¹, Jing Wang³

¹State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, No. 651, Dongfeng Road East, Guangzhou, 510060, P. R. China.

Journal of translational medicine

|November 11, 2025

概括

截断的腺多样性大肠杆菌 (APC) 蛋白质抑制了结直肠癌 (CRC) 的瘤免疫力. 移除Trunc-APC并使用表观遗传疗法可以增强抗瘤反应并抑制CRC生长.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

突变腺瘤性多样性大肠杆菌 (APC) 基因产生截断APC (Trunc-APC),作为结直肠癌 (CRC) 的瘤基因.
在调节瘤细胞天生的免疫反应中Trunc-APC的作用基本上是未知的.

研究的目的:

研究Trunc-APC在CRC中的免疫调节功能.
确定Trunc-APC如何影响天生的免疫力及其作为治疗点的潜力.

主要方法:

在CRC细胞系中,CRISPR-Cas9基因编辑以淘汰突变APC.
转录组测序,亚细胞分离和蛋白质相互作用研究.
在体外和体内评估Trunc-APC缺失对免疫激活,亡和瘤生长的影响,单独和与表观遗传剂一起.

主要成果:

Trunc-APC定位在线粒体外膜上,结合MAVS抑制I型干扰素信号传递并破坏RIG-I相互作用.
Trunc-APC删除增强了先天性免疫激活和瘤细胞亡.
结合Trunc-APC删除与表观遗传疗法 (5-azacytidine和trichostatin A) 显著抑制了CRC瘤的生长.

结论:

在CRC中,Trunc-APC在抑制瘤内在的先天免疫力方面发挥着新的作用.
与表观遗传疗法共同准Trunc-APC是一种有希望的策略,可以增强CRC的抗瘤免疫力.

关键词:

结肠直肠癌是一种癌症.天生的免疫系统天生的免疫系统.马夫斯 (MAVS) 是一种动物.在Trunc-APC中使用.第一种类型的干扰子信号传输.

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