基于深度学习的核细分特征的融合,用于微卫星不稳定性和在消化道癌症中预测瘤突变负担:一个多中心验证研究
Yanping Zhang1, Jiaying Han1, Huang Chen2
1School of Mathematics and Physics, Hebei University of Engineering, 19 Taiji Road, Handan 056038, China.
Briefings in bioinformatics
|November 11, 2025
概括
这项研究引入了一种深度学习方法,用于从H&E染色的幻灯片中预测微卫星不稳定性 (MSI) 和瘤突变负担 (TMB),为胃癌和结肠直肠癌的基因组测试提供了具有成本效益的替代方案.
科学领域:
- 计算病理学计算病理学
- 数字病理学数字病理学
- 在瘤学瘤学.
背景情况:
- 微卫星不稳定性 (MSI) 和瘤突变负担 (TMB) 是胃 (GC) 和结直肠癌 (CRC) 免疫治疗反应的关键生物标志物.
- 目前基于测序的检测方法昂贵且耗时,这限制了它们的临床实用性,因为只有一部分患者受益.
研究的目的:
- 开发和验证一个深度学习框架,直接从血素和素 (H&E) 染色的整片图像中预测MSI和TMB状态.
- 调查融合核细分特征的实用性,以提高预测准确度.
主要方法:
- 从TCGA数据集 (GC和CRC) 中使用CLAM提取图像特征和使用Hover-Net提取核特征.
- 通过多模式紧双线聚合结合了功能,并训练了六个深度学习模型.
- 在CRC数据集上使用五倍交叉验证和外部验证评估模型性能.
主要成果:
- 与仅使用图像模型相比,集成核细分特征的深度学习模型提高了AUC1% - 3%和回忆率5% - 11%.
- 外部验证在CRC中实现了MSI预测的AUC为0.81和回忆为0.80.
- 在癌症类型和临床群体中观察到细胞组成的显著差异.
结论:
- 将H&E图像功能与核细分数据和深度学习相集成,为MSI/TMB预测提供了具有成本效益和准确的方法.
- 这种方法有可能减少对昂贵的基因组测试的需求,推进个性化癌症治疗策略.
相关概念视频
Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...


