Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Using rotational integration of oblique interferometric scattering to track axial spatiotemporal responses of tubular membrane protrusions.

Nature communications·2026
Same author

Superconducting nanowire single-photon detectors for enhanced biomedical imaging.

Journal of biomedical optics·2026
Same author

Quantifying DNA damage following light sheet and confocal imaging of the mammalian embryo.

Scientific reports·2024
Same author

Viewing early life without labels: optical approaches for imaging the early embryo†.

Biology of reproduction·2024
Same author

Minimally invasive longitudinal intravital imaging of cellular dynamics in intact long bone.

Nature protocols·2023
Same author

Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption.

Cell·2021

相关实验视频

Updated: Jan 11, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.0K

结合多位点FRAP和HILO-TIRF显微镜,使用空间光调节器.

Avinash Upadhya, Yean Jin Lim, Woei Ming Lee

    Optics express
    |November 11, 2025
    PubMed
    概括

    这项研究引入了一种新的多位置光白化后恢复 (FRAP) 技术. 它可以在动态的生物环境中同时进行高对比成像和运动研究,克服现有方法的局限性.

    科学领域:

    • 生物物理学的生物物理.
    • 细胞动力学细胞动力学
    • 显微镜技术 显微镜技术

    背景情况:

    • 光漂白后光恢复 (FRAP) 对于研究细胞内动力学至关重要.
    • 目前的FRAP系统面临着由于度扫描的同时多位置分析和高级成像模式的局限性.
    • 这些局限性阻碍了对动态和非同位素生物环境的研究.

    研究的目的:

    • 开发一种通用的FRAP技术,使同时进行多位置分析.
    • 将高对比度成像模式 (HILO,TIRF) 与多位置FRAP集成在一起.
    • 在现有的FRAP系统中克服时间空间多重复合的权衡.

    主要方法:

    • 使用了带有弗雷内尔透镜和衍射面具的相位配置文件,用于空间复杂化.
    • 实现了单个空间光调节器来控制多个FRAP位置.
    • 集成的光,高度倾斜的层状光学板 (HILO) 和总内部反射光 (TIRF) 显微镜.

    主要成果:

    • 实现了独立空间位置的同时FRAP.
    • 保持了高对比度成像模式 (HILO和TIRF) 以及多位置FRAP.
    • 启用了高对比度漂白和跨体积的选.

    更多相关视频

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.3K
    A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
    16:10

    A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

    Published on: March 22, 2012

    24.4K

    相关实验视频

    Last Updated: Jan 11, 2026

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    26.0K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.3K
    A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
    16:10

    A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

    Published on: March 22, 2012

    24.4K

    结论:

    • 开发的技术提供了高对比度成像的同时多位置FRAP.
    • 这种多功能仪器对于单粒子跟踪和单分子成像非常有价值.
    • 方便在动态生物系统中跨视野测量快速事件.