Clba基因的分布及其与MDR大肠杆菌中抗菌素耐药性模式的相关性来自不同宿主群体的E. coli
Soma Kanta Baral1,2, Govardhan Joshi2, Indira Parajuli3
1Central Department of Biotechnology, Tribhuvan University, Kathmandu, Nepal.
MicrobiologyOpen
|November 11, 2025
概括
具有编码大肠杆菌素的clbA基因的多药耐药性大肠杆菌 (大肠杆菌) 在临床患者中更为普遍,并且对临床关键的抗生素如伊米和美罗具有更高的耐药性.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 公共卫生 公共卫生
背景情况:
- 多抗药性大肠杆菌 (E. coli) 构成了严重的公共卫生威胁.
- 负责编码基因毒素大肠杆菌素的clbA基因是大肠杆菌中关键的毒性因子.
研究的目的:
- 调查clbA基因在不同种群的MDR大肠杆菌分离物中的患病率.
- 检查 clbA 基因存在与 MDR E. coli 中的抗菌素耐药性模式之间的关联.
主要方法:
- 从尼泊尔两个医疗保健中心的正常人,癌症患者和临床患者中收集了115个MDR大肠杆菌分离物.
- 利用分子方法检测clbA基因的存在.
- 分析了分离物的抗菌素耐药性模式.
主要成果:
- 在13%的MDR大肠杆菌分离物中检测到clbA基因.
- 与癌症患者 (8.6%) 和正常人 (5.0%) 相比,临床患者 (25.0%) 的clbA基因患病率显著更高.
- 与clbA阴性分离物相比,clbA阳性分离物对伊米 (100%),美罗 (100%) 和阿米卡辛 (100%) 的耐药性显著增加.
结论:
- 在MDR大肠杆菌中存在编码大肠杆菌素的clbA基因与抗菌素耐药性增加有关.
- 在临床环境中更高的患病率表明在临床感染中可能发挥作用.
- 强调需要针对MDR大肠杆菌携带毒性基因的分子监测和感染控制策略.
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