Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.6K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.6K
DNA Bacteriophages01:26

DNA Bacteriophages

773
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
773
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K
Methods of Classification and Identification01:28

Methods of Classification and Identification

972
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
972
Special Staining Techniques01:13

Special Staining Techniques

1.1K
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
1.1K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

606
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
606

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Dynamic adhesion device of phage OE33PA drives Gram-positive host recognition.

bioRxiv : the preprint server for biology·2026
Same author

Periodontal health in a longitudinal French cohort of people with Parkinson's disease.

Scientific reports·2026
Same author

Characterization of strictly lytic phages infecting <i>Oenococcus oeni</i> from Merlot wines and proposal of a new genus.

Microbiology spectrum·2025
Same author

Structural and Genetic Diversity of Lysis Modules in Bacteriophages Infecting the Genus <i>Streptococcus</i>.

Genes·2025
Same author

The great phage escape: Activating and escaping lactococcal antiphage systems.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Insight into crRNA Processing in <i>Streptococcus mutans</i> P42S and Application of SmutCas9 in Genome Editing.

International journal of molecular sciences·2025

相关实验视频

Updated: Jan 11, 2026

Purification of a High Molecular Mass Protein in Streptococcus mutans
09:51

Purification of a High Molecular Mass Protein in Streptococcus mutans

Published on: September 14, 2019

6.3K

在Streptococcus mutans菌株中防御系统和食原体检测菌株

Olivier Claisse1, Cas Mosterd1, Claire Le Marrec1

  • 1University of Bordeaux, INRAE, Bordeaux INP, Bordeaux Sciences Agro, UMR 1366, OENO, ISVV, Villenave d'Ornon, France.

Molecular oral microbiology
|November 11, 2025
PubMed
概括

Streptococcus mutans 具有多样化的抗菌防御系统 (APDS),主要是限制修饰系统. 研究人员在CRISPR-Cas系统中发现了新的变异和独特的prophage,为细菌适应菌体压力提供了洞察力.

关键词:
突变型链球菌 (Streptococcus mutans) 是一种菌.生物信息学是一种生物信息学.免疫学 免疫学 免疫学

更多相关视频

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
12:06

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Published on: October 23, 2017

8.1K
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

25.1K

相关实验视频

Last Updated: Jan 11, 2026

Purification of a High Molecular Mass Protein in Streptococcus mutans
09:51

Purification of a High Molecular Mass Protein in Streptococcus mutans

Published on: September 14, 2019

6.3K
Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
12:06

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Published on: October 23, 2017

8.1K
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

25.1K

科学领域:

  • 微生物学 微生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 关于Streptococcus mutans的抗菌体防御系统 (APDS) 的数据有限.
  • S. mutans被广泛研究,但其菌体防御机制尚未得到充分探索.

研究的目的:

  • 探索临床S. mutans分离物中APDS的多样性和发生情况.
  • 使用生物信息学工具,在这些分离物体的基因组中识别菌体.

主要方法:

  • 来自44个临床S. mutans分离物的基因组DNA被测序.
  • 使用DefenseFinder,PADLOC,CRISPRCasFinder和PhiSpy来分析APDSs和prophages. 这三种类型的数据被用于分析APDSs和prophages.
  • 在AcrFinder和AcrHub中,他们发现了抗CRISPR蛋白.

主要成果:

  • 每个S. mutans菌株都含有6-12个APDS,其中限制修饰系统是最常见的.
  • 在II-A型Cas9蛋白中发现了新的变异,80%的间隔器针对未知的实体.
  • 检测到一种独特的prophage,phi_37bPJ2,编码了一个保存的抗CRISPR蛋白 (AcrIIA5).

结论:

  • S. mutans 呈现出多种不同的 APDS,其中 prophages 的存在有限.
  • 这些发现为了解S. mutans适应菌体压力和APDS演变提供了基础.