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Updated: Jan 11, 2026

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
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Published on: February 17, 2023

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在CRISPR-Cas9中扩展PAM兼容性的设计规则来自VQR,VRER和EQR变体

Francisco Vieyra1, Chinmai Pindi2,3, George P Lisi4,5

  • 1Department of Inorganic, Analytical & Chemical Physics, University of Buenos Aires, Buenos Aires C1053ABH, Argentina.

The journal of physical chemistry. B
|November 11, 2025
PubMed
概括

扩展CRISPR-Cas9基因组编辑需要了解它如何识别原生空间器相邻动机 (PAMs). 这项研究揭示了PAM识别涉及到局部稳定,远距离合和入调节,以提高编辑效率.

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