随着衰老和男性大鼠骨肌肉的卡路里限制而发生的miRNA变化:肌肉细胞功能中的潜在作用
Gulam Altab1, Brian J Merry2, Charles W Beckett1
1Integrative Genomics of Ageing Group, Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, L7 8TX, UK.
Biogerontology
|November 11, 2025
概括
骨肌肉的衰老涉及改变的微RNA (miRNA) 表达. 热量限制 (CR) 部分扭转了这些变化,影响了肌肉发育,新陈代谢和炎症途径.
科学领域:
- 分子生物学分子生物学
- 老年学是指老年学的学科.
- 骨肌肉生理学 骨肌肉生理学
背景情况:
- 骨肌肉衰老的机制尚不清楚.
- 失调的微 (mi) RNA表达与肌肉衰老有关.
- 众所周知,热量限制 (CR) 会影响衰老过程.
研究的目的:
- 在老鼠骨肌中全面研究与年龄相关的miRNA表达变化.
- 研究CR对这些与年龄相关的miRNA变化的影响.
- 为了确定改变miRNA表达对向蛋白质和途径的功能后果.
主要方法:
- 年轻和老老鼠骨肌肉的高通量RNA测序.
- 对miRNA表达特征的分析和与年龄和CR相关的miRNAs的识别.
- 生物信息分析用于预测蛋白质标和相关途径.
- 在体外研究中使用C2C12细胞核细胞来评估miR-96-5p的功能.
主要成果:
- 确定了许多与年龄相关的miRNAs;CR正常化超过35%到年轻人的水平.
- 上调的miRNAs向参与肌肉发育,新陈代谢和寿命的蛋白质 (例如AMPK,自).
- 下调的miRNAs向促炎性蛋白质,包括先天免疫,补充和凝血级联中的蛋白质.
- 在正常化上调 miRNA 的过程中,CR 的效果比下调 miRNA 的效果更高.
- 随着年龄的上升,miR-96-5p的上调降低了细胞活力,线粒体生物发生,肌原分化和C2C12细胞的自.
结论:
- 随着骨肌肉的衰老,miRNA表达发生显著的变化.
- 这些miRNA变化影响了包括新陈代谢,发育,寿命和炎症在内的关键细胞过程.
- CR部分改善了与年龄相关的miRNA失调和相关的功能后果.
- 像miR-96-5p这样的特定miRNA在肌肉衰老过程中发挥着功能性作用.
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