来自菌体DCp1的内素LysDCp1的高化活性对抗Clostridium perfringens和生物膜
Ruixue Ma1, Zhaohui Tang1,2, Jianchao Zhao1
1College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
Veterinary research communications
|November 11, 2025
概括
一种新型的菌体内溶素,LysDCp1,对Clostridium perfringens表现出强烈的活性,为治疗动物腹和减少经济损失提供了抗生素的有希望的替代品.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 动物卫生 动物健康
背景情况:
- 菌会导致动物,特别是年轻动物的严重死亡和经济损失.
- 抗生素耐药性需要替代性治疗细菌感染.
- 菌体及其衍生的内素正在成为抗生素的可行的替代品.
研究的目的:
- 从菌体DCp1.1.中表达和净化内解素LysDCp1.
- 为了评估LysDCp1对Clostridium perfringens的溶解活性和杀菌效果.
- 在活体动物模型中评估LysDCp1的治疗潜力.
主要方法:
- 恩多利辛LysDCp1的表达和净化.
- 在体外溶解活性测定对抗C. perfringens菌株.
- 最低抑制度 (MIC) 的确定.
- 生物膜消除试验.生物膜消除试验.
- 在体内使用Galleria mellonella幼虫模型进行有效性测试.
主要成果:
- 莱斯DCp1对C. perfringens表现出广泛的溶解活性,超过了父菌体的活性.
- 有效降低C. perfringens D14度,其MIC为12.5μg/mL.
- 在中性pH值和中等温度下最佳活性;在高温和性条件下稳定.
- 在3小时内,C. perfringens生物膜的显著消除.
- 在G. mellonella幼虫中显著降低了细菌负载和改善了生存率.
结论:
- 莱斯DCp1是一种强大的抗菌剂,可以对抗Clostridium perfringens.
- LysDCp1 具有良好的稳定性和广泛的活性.
- LysDCp1 显示出在动物中治疗密闭性腹的显著治疗潜力.
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