在番茄果实成熟过程中,SlaBF4作为乙烯生物合成的负调节剂
Xiawan Zhai1,2, Xiaoqing Gao1, Jinran Huang1
1Jiangxi Provincial Key Laboratory for Postharvest Storage and Preservation of Fruit and Vegetables, Jiangxi Agricultural University, Nanchang, 330045, China.
The Plant journal : for cell and molecular biology
|November 11, 2025
概括
SlABF4通过直接抑制乙烯生物合成基因来负面调节番茄果的成熟. 这个转录因子是转录因子.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- ABF/AREB转录因子对于植物应激反应和发育至关重要.
- 对于ABF/AREB蛋白在水果成熟,特别是番茄中的作用,尚不清楚.
研究的目的:
- 阐明SLABF4在番茄果实成熟中的功能和调节机制.
- 研究SLABF4与其他成熟调节者的分子相互作用.
主要方法:
- 产生SLABF4过度表达 (OE) 线和CRISPR/Cas9淘汰 (CR) 突变物.
- 对水果成熟表型,乙烯生产和基因表达 (转录组,qRT-PCR) 的分析.
- 分子测试包括酵母一混合 (Y1H),双路西法酶记者测试和电泳运动转移测试 (EMSA).
主要成果:
- 在番茄果实成熟期间,slABF4的表达减少.
- OE-SlABF4系表现出延迟的成熟和减少的乙烯,而CR-slabf4突变体表现出加速的成熟和增加的乙烯.
- SlABF4直接与 SlACS2 和 SlACS12 促进体结合,抑制乙烯生物合成,并与 SlFUL1 和 SlMADS1.1 相互作用.
结论:
- SlABF4作为番茄果实成熟的负调节剂.
- SlABF4通过直接抑制关键基因和与其他转录因子相互作用来调节乙烯生物合成.
- 研究结果为精确的育种策略提供了洞察力,以控制番茄果实成熟.
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