蝙蝠merbecoviruses中的新型ACE2结合扩大了潜在宿主范围
Xiaoguang Zhang1, Xing Ma1, Ye Chen2
1Academy for Advanced Interdisciplinary Studies, Engineering Laboratory of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
PLoS pathogens
|November 11, 2025
概括
这项研究揭示了蝙蝠merbecoviruses如何与ACE2受体结合,一些病毒显示了广泛的宿主范围,包括潜在的中间宿主,如子和松鼠,突出了动物传播的风险.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 生态生态学 生态生态学
背景情况:
- 冠状病毒表现出由受体结合决定的宿主特异性,影响动物感染潜力.
- 梅尔贝病毒 (例如,MERS-CoV) 使用DPP4,而沙尔贝病毒 (例如,SARS-CoV) 使用ACE2.
- 蝙蝠传播的美贝病毒构成潜在的动物传染性威胁.
研究的目的:
- 调查四种蝙蝠merbecoviruses的ACE2受体使用情况:HKU5,BtVs-SC2013,HKU25和P. khulii-2011.
- 阐明物种特异性ACE2结合和宿主适应的基础分子机制.
- 评估跨物种传播给非蝙蝠宿主的可能性.
主要方法:
- 针对蝙蝠merbecoviruses的特定物种ACE2结合测定.
- 对ACE2-RBD相互作用的结构分析和位点定向突变发生.
- 研究糖化位点对受体结合的影响.
主要成果:
- HKU5显示出对Pipistrellus abramus ACE2的排他性结合,而P. khulii-2011只结合Murina aurata ACE2. 这种结合是非常明显的.
- BtVs-SC2013与Murina aurata和Myotis myotis ACE2结合,并且还与子 ACE2结合.
- HKU25表现出广泛的ACE2结合,包括松鼠和子ACE2,这表明潜在的中间宿主.
- 在P. abramus ACE2上,一个特定的N-糖化位点对HKU5结合至关重要;它的修改会影响结合.
- 在子ACE2中发现了关键的残留物,用于BtVs-SC2013和HKU25结合.
结论:
- 蝙蝠merbecoviruses显示不同的ACE2受体热带和宿主特异性.
- 结合机制涉及特定的ACE2残留物和糖化模式.
- 这些发现有助于更好地了解蝙蝠merbecovirus的演变和动物传播传播潜力.
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