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在法医样本分析中应用DBS/LC-MS方法

Alicja Wieczorek¹, Karolina Mrochem², Rafał Skowronek²

¹Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University in Kraków, 2 Gronostajowa St., Kraków 30-387, Poland.

Forensic science international

|November 11, 2025

概括

干血斑点 (DBS) 卡片为在死后毒理学中检测精神活性物质提供了一种有效的方法. 这种优化的DBS/LC-MS技术提供了与传统方法相比可靠的结果,有助于法医调查.

科学领域:

法医毒理学法医毒理学
分析化学分析化学
药理学药理学是指药理学的学科.

背景情况:

干血斑 (DBS) 卡因其效率越来越高,在医学和毒理学中越来越多地使用.
传统的死后血液分析方法面临的挑战是样本的可用性和储存.
优化样本准备对于提高法医科学中DBS卡的实用性至关重要.

研究的目的:

开发和验证一个全面的干血斑 (DBS) 液态染色体质谱法 (LC-MS) 方法.
在死后血液样本中检测16种精神活性物质.
将DBS/LC-MS方法与已建立的LC-Selected Reaction Monitoring-Mass Spectrometry (LC-SRM-MS) 技术进行比较.

主要方法:

在DBS卡片的样本准备过程中提升了提取和消除过步骤.
验证DBS/LC-MS方法的精度,可重复性和灵敏度.
对DBS/LC-MS结果与LC-SRM-MS结果的比较分析使用死后血液样本.

主要成果:

通过优化提取,提高检测极限 (LOD) 来实现分析剂度的十二倍增加.
经过验证的DBS/LC-MS方法显示出高精度,可重复性和对精神活性物质的敏感性.
DBS/LC-MS结果与LC-SRM-MS一致,为特定分析物提供较低的LOD.

结论:

优化的DBS/LC-MS方法是法医毒理学的可靠而敏感的工具.
DBS卡提供优势,例如减少样本量和成本效益高的储存,使其成为毒理学分析的有价值替代品.
常规使用DBS卡可以解决死后样本供应方面的挑战,并帮助及时进行法医调查,特别是在波兰等地区.

关键词:

干燥的血液斑点干燥的血液斑点法医毒理学法医毒理学液体色谱学液体色谱学液体色谱学尸体解剖后进行分析.

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