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矩阵刚度调节人角膜内皮细胞中YAP介导的糖解和增殖

Mingyue Zhong¹, Jun Zhao¹, Tingjun Fan¹

¹Key Laboratory for Corneal Tissue Engineering, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, Shandong Province, China.

Biomaterials advances

|November 11, 2025

概括

脚手架硬度调节人角膜内皮 (HCE) 细胞的增殖和新陈代谢. 增加的刚性通过YAP途径增强细胞生长和糖解,这对于组织工程至关重要.

科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
细胞生物学细胞生物学

背景情况:

人类角膜内皮 (HCE) 对于角膜透明度至关重要;细胞密度降低导致视力丧失.
供体角膜的稀缺性限制了治疗方法,突出了组织工程HCE (TE-HCE) 的需要.
脚手架的机械微环境是TE-HCE发育的关键,影响细胞增殖和新陈代谢.

研究的目的:

研究矩阵刚度在调节HCE细胞代谢和增殖中的作用.
为了探索模拟Descemet的膜的聚烯胺水凝的机械性能.
阐明脚手架刚性,YAP信号和细胞代谢途径之间的关系.

主要方法:

使用不同硬度 (25,50,100kPa) 的聚烯胺基凝来模仿Descemet的膜.
评估HCE细胞的增殖,代谢活动和基因表达,以应对刚性.
分析了与YAP相关的蛋白质 (YAP) 核转位和糖解路径调节.

主要成果:

增加的支架刚度显著促进了HCE细胞的增殖.
增强的刚性导致YAP核转位和下游扩散基因表达的增加.
较高的刚度提高了HCE细胞中的糖解调节,提高了生命力和能量生产.
脚手架的刚性影响了通过YAP和糖解的增殖,建立了一个积极的反循环.

结论:

矩阵刚度是HCE细胞增殖和新陈代谢的关键调节者.
YAP途径调解了硬度对细胞增殖和糖解的影响.
这些发现为开发具有用于角膜再生的优化机械性能的TE-HCE提供了基础.

关键词:

细胞的增殖细胞的增殖.葡萄糖溶解是什么? 葡萄糖溶解代谢反应的反应聚烯胺的水凝.组织工程的人类角膜内皮

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