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Updated: Jan 11, 2026

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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低温EM和计算建模对pH调节器NBCn1的结构洞察力

Weiguang Wang1,2, Hristina R Zhekova3, Kirill Tsirulnikov1

  • 1Department of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.

Nature communications
|November 11, 2025
PubMed
概括

乳腺癌细胞通过NBCn1转运器在酸性条件下生存. 结构和功能研究显示,高效的电梯机制和高的循环率有助于癌细胞的生存.

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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