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在小麦中存在的CONSTANS类基因家族涉及标题日期和干旱应激反应
Bentolhoda Farokhpouri1, Ghader Mirzaghaderi2, Adel Siosemardeh1
1Department of Plant Production and Genetics, Faculty of Agriculture, University of Kurdistan, Postal Code: 6617715175, Sanandaj, Iran.
Scientific reports
|November 11, 2025
概括
这项研究确定了小麦中的51个CONSTANS-like (COL) 基因,揭示了它们对干旱反应的作用. 在干旱期间,某些COL基因受到上调,特别是在弹性小麦系中,这表明它们对适应的重要性.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 遗传学 是一个遗传学.
背景情况:
- 康斯坦斯类 (COL) 指转录因子通过将昼夜节律与花诱导联系起来来调节开花时间.
- 在非生物应激反应,特别是干旱中,COL基因的参与尚未得到充分理解.
研究的目的:
- 为了识别和描述普通小麦 (Triticum aestivum) 中的 COL 基因家族.
- 研究TaCOL基因在应对干旱压力的表达模式和潜在作用.
- 探索TaCOL基因表达与小麦干旱的生理适应之间的关系.
主要方法:
- 在小麦中的51个COL基因的识别和遗传学分析.
- 发起人分析以识别 cis 监管要素.
- 对RNA测序数据集的分析,以确定TaCOL基因表达特征.
- 在干旱压力下进行水培实验和生理分析.
- 定量PCR (qPCR) 用于验证基因表达.
主要成果:
- 确定了51个TaCOL基因,显示染色体分布不均,并分为9个家族遗传分类.
- 促成体分析揭示了TaCOL基因中对光和酸反应的元素.
- 大多数TaCOL基因都在各种组织中表达,具有偏好的叶子表达.
- 一些TaCOL基因,包括TaCOL-12/16/24/51,表现出干旱反应.
- 干旱压力对TaCOL基因表达产生了不同的影响,其中一些基因受到上调,而另一些基因受到下调.
- 早期成熟的合成小麦系 (R16P1) 在干旱下表现出较少的严重生理损伤,与其父系相比,某些TaCOL基因的表达更高.
结论:
- 塔科尔基因家族在小麦适应干旱压力方面发挥着重要作用.
- 特定的TaCOL基因受到干旱的差异调节,并与生理弹性有关.
- 塔科尔基因代表了基因改进的潜在目标,以提高小麦的早熟性和耐旱性.
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