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通过冷大气等离子体喷射增强Komagataella phaffii的重组陶马丁的产生

Nivedita Parsekar¹, Simen Akkermans¹, Dmytro Kozak¹

¹BioTeC+, Chemical and Biochemical Process Technology and Control, KU Leuven, Gebroeders de Smetstraat 1, Ghent, B-9000, Belgium.

Scientific reports

|November 11, 2025

概括

冷大气等离子体治疗显著增强了酵母中的重组蛋白质生产13.3%. 这种非热方法提高了每消耗的甲醇量中陶马丁 (一种蛋白质) 的产量,表明了更有效的生物处理.

科学领域:

生物技术是生物技术.
生物工艺工程生物工艺工程
血科学是一门科学课.

背景情况:

冷大气等离子体 (CAP) 为调节微生物新陈代谢提供了一种新的,非热和非侵入性方法.
由于其在生物反应器中增强生物生产过程的潜力,CAP越来越多地被探索.

研究的目的:

为了验证冷大气等离子喷射对重组陶马丁生物生产的影响.
为了研究CAP治疗时间对转基因Komagataella phaffii.的thaumatin产量的影响.

主要方法:

经过基因改造的Komagataella phaffii被培养使用甲醇作为碳源.
无细胞介质和每日的细胞培养被用等离子喷射处理,持续时间不同 (2-8分钟).
重组陶马丁产量和残留甲醇度被量化并与未经处理的对照进行比较.

主要成果:

与对照组相比,CAP治疗无论持续时间如何,都显著提高了总体蛋白质产量13.3%.
血治疗的持续时间和thaumatin生产水平之间没有发现显著的相关性.
血治疗增加了每单位消耗的甲醇的thaumatin的产量,这表明改善了代谢效率.

结论:

冷大气等离子体的应用明显增强了Komagataella phaffii中的重组蛋白生物生产.
这项研究支持了CAP产生的反应性物种在提高蛋白质产量方面发挥关键作用的假设.
CAP提出了一个有前途的战略,用于优化生物过程,提高蛋白质生产的效率.

关键词:

这就是Pichia pastoris.大气中的冷等离子体.生物生产是一种生物生产.甲醇是甲醇中的一种.再组合蛋白质是一种重组蛋白质.陶马丁是一种酸盐.

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