在Pogostemon cablin中,PcMYB25的功能性表征是帕丘利酒精生物合成的候选调节者,通过外源激素诱导的转录组分析,通过外源激素诱导的转录组分析
Liqiong Feng1, Xuanxuan Zhou1, Peihan Chen1
1Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Key Laboratory of Chinese Medicinal Resource from Lingnan (Guangzhou University of Chinese Medicine), Ministry of Education, Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines, Guangzhou, 510006, P. R. China.
BMC plant biology
|November 11, 2025
概括
植物激素通过激活PcMYB25转录因子来调节帕丘利酒精 (PA) 的产生. 这一发现增强了对甲类生物合成的理解,并为PA生产提供了新的策略.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 植物激素会影响二次代谢物的产生,包括Pogostemon cablin中的帕丘利酒精 (PA).
- 虽然已知PA生物合成途径,但其涉及转录因子的调节机制尚未完全理解.
研究的目的:
- 研究帕丘利酒精生物合成的调节机制,以应对植物激素治疗.
- 确定参与荷尔蒙介导的PA生产调节的关键转录因子.
主要方法:
- 转录组分析以评估激素治疗后的基因表达变化.
- 权重基因共同表达网络分析 (WGCNA) 以确定候选调节基因.
- 过渡性过度表达,酵母单杂交和转录激活试验用于验证基因功能.
主要成果:
- 外源激素的应用显著改变了Pogostemon cablin的基因表达,特别是在类代谢中.
- PcMYB25,一个R2R3-MYB转录因子,被确定为一个关键的调节器.
- 过度表达PcMYB25增加了85%的PA含量,调高了PcPTS和MVA通路基因,并证明了直接与PcPTS促进体结合.
结论:
- PcMYB25作为植物激素信号传递的积极调节剂,促进PA生物合成.
- 这项研究提供了对PA生产的转录调节和基因工程策略的潜力的见解.
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