葡萄糖诱导的活跃 lysogeny 的prophage 的 ΦSa3XN 促进金黄色葡萄球菌的毒性
Wei Huang1, Xing Tian1, Keting Zhu1
1Department of Emergency Medicine, the Second Affiliated Hospital of Army Medical University, Chongqing, China.
Virology journal
|November 11, 2025
概括
这项研究表明,葡萄糖触发了黄金葡萄球菌中Sa3int菌体的切除,增强了细菌的毒性. 这种由CcpA调节的活性 lysogeny 机制,会影响黄金色杆菌感染的结果.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 病毒学 病毒学
背景情况:
- 活性 lysogeny 涉及菌体的整合和切除作为调节细菌基因表达的分子开关,而不会产生新的病毒.
- 在黄金葡萄球菌中普遍存在的圣菌体,集成到β毒素基因 (hlb) 中,感染条件促进菌体损失和 Hlb 生产.
- 控制Sa3int菌体活性溶解的环境线索和机制在很大程度上是未知的.
研究的目的:
- 为了研究Staphylococcus aureus中Sa3int菌体的活性 lysogeny.
- 确定环境线索和调节Sa3int菌体生命周期的分子机制及其对细菌毒性的影响.
主要方法:
- 来自MRSA菌株XN108.8的Sa3int菌体 (ΦSa3XN) 的鉴定和表征.
- 使用PCR,斑块试验,TEM和DNase保护试验分析活性 lysogeny.
- 评估葡萄糖诱导的活性溶解发生及其对黄金色杆菌毒性的影响,通过记者测试,EMSA,血液溶解测试和小鼠感染模型.
主要成果:
- ΦSa3XN表现出活跃的溶解生成,在没有后代产生的情况下切除,作为分子开关起作用.
- 葡萄糖作为环境暗示剂,诱导 ΦSa3XN 切除并通过 CcpA 介导的 CI 抑制剂抑制恢复 hlb 表达.
- 葡萄糖诱导的活性 lysogeny 显著增加黄金色球菌的溶血活性,恶化皮肤炎症和在高血糖小鼠.
结论:
- 这项研究呈现了Sa3int菌体中活跃 lysogeny 的新奇实例.
- 一个对葡萄糖敏感的CcpA通路调节了FSa3XN的切除,增强了金黄色葡萄球菌的毒性.
- 这些发现促进了对S. aureus-phage相互作用和活性 lysogeny机制的理解.
相关概念视频
Lysogenic Cycle of Bacteriophages
67.3K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
67.3K
Viral Replication: Lysogenic Cycle
1.3K
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
1.3K
Gene Regulation in Microbial Communities: Quorum Sensing
486
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
486
Operons
53.9K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
53.9K
Prokaryotic Transcriptional Activators and Repressors
25.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
25.1K
DNA Bacteriophages
773
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
773


