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在快速分裂的瘤细胞中通过非线性磁化方法检测生物性磁性纳米粒子

Marina V Milovanova^1,2, Anna N Gabashvili¹, Elizaveta N Mochalova^3,4

¹Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia.

Frontiers in bioengineering and biotechnology

|November 12, 2025

概括

遗传编码的细菌纳米区 (encapsulins) 能够在哺乳动物细胞中产生磁性纳米粒子. 这种方法可以通过磁粒子量化来对细胞增殖进行敏感的实时监测.

科学领域:

生物医学是生物医学.
生物技术是生物技术.
纳米技术纳米技术

背景情况:

细菌封装素是具有生物医学应用的基因编码纳米平台.
热耐受性菌 (Qt) 封装剂系统产生具有T2对比性质的磁纳米粒子 (MNP).
设计以表达Qt基因的哺乳动物细胞可以使用MNP产生并被检测出来.

研究的目的:

研究用Qt封装素标记哺乳动物细胞的优点和局限性.
评估Qt封装素用于细胞跟踪和监测的使用.
评估磁粒子量化 (MPQ) 对实时细胞增殖研究的潜力.

主要方法:

设计了一种老鼠C6质瘤细胞系,以表达红色光蛋白 (RFP) 和Qt纳米区.
使用ICP-MS,Perls染色,MPQ,光显微镜和光学断层扫描来表征细胞.
使用严重综合免疫缺陷 (SCID) 的小鼠进行了体内研究.

主要成果:

在工程哺乳动物细胞中证明了MNP的成功产生.
使用MPQ的C6-RFP-Qt细胞中估计的磁信号保留时间.
展示了在细胞增殖过程中对磁信号振幅实时监测的潜力.

结论:

Qt封装剂为标记具有磁性特性的哺乳动物细胞提供了一个有前途的工具.
该MPQ方法为研究MNP和细胞增殖提供了一个敏感而简单的替代方案.
这种方法增强了质母细胞瘤多样性研究的体内模型.

关键词:

生物的纳米粒子是生物的.囊素是一种囊素.在体外和体外监测监测.磁性纳米粒子是一种磁性纳米粒子.磁性颗粒量化测量磁性粒子量化测量多模式检测多模式检测这是光学成像.基于蛋白质的纳米材料

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