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Updated: Jan 11, 2026

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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一种基于CRISPR的实用方法,用于快速编辑Caulobacter crescentus的基因组.

Xuezhou Yuan1,2,3, Xin Yu1,2, Wei Zhao1,2

  • 1State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Bio-protocol
|November 12, 2025
PubMed
概括

这项研究引入了一种CRISPR/SpCas9M报告系统,用于高效的基因组编辑,用于像Caulobacter crescentus这样的反抗性细菌. 这种新系统可以在一周内快速删除,减少基因标记物和其他修改.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?这是一种Caulobacter crescentus.基因组编辑 基因组编辑报告系统报告系统在SpCas9M中使用.

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