唾液和鼻子前部样本用于检测儿童呼吸道病毒
Anu Haaramo1, Anu Jääskeläinen2, Anne Pitkäranta1
1Department of Otorhinolaryngology-Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, PO, Finland.
前鼻是使用聚合酶链反应 (PCR) 测试检测儿童呼吸道病毒的鼻口腔的舒适而准确的替代品.
科学领域:
- 儿童传染病 儿童传染病
- 分子诊断学 分子诊断学
- 呼吸道病毒学 呼吸道病毒学
背景情况:
- 鼻 swab 是通过聚合酶链反应 (PCR) 检测呼吸系统病毒的标准.
- 需要更少的侵入性采样方法,特别是对于儿科患者.
- 前鼻腔和唾液样本已被提出作为替代方案.
研究的目的:
- 为了比较前鼻和唾液样本的准确性和耐受性与鼻和喉的拭子,用于儿童的呼吸道病毒检测.
- 评估前鼻作为小儿呼吸道病毒PCR检测的主要采样方法的可行性.
主要方法:
- 一项前性研究,涉及59名患有呼吸道症状的儿科患者.
- 使用多重呼吸板和四重PCR进行前鼻拭子,唾液样本和鼻拭子的结果的比较.
- 对儿童样本采集耐受性的评估.
主要成果:
- 之前的鼻腔和唾液样本采集与鼻口腔抽样相比,儿童可以更好地容忍.
- 前鼻腔样本在检测呼吸道病毒方面比唾液样本更准确.
- 多重PCR分析证实了相对于鼻参考标准的前鼻拭子的诊断性能.
结论:
- 前鼻是一种耐受性良好的,准确的替代品,用于检测有症状的儿童的呼吸道病毒.
- 前鼻抽样是一种可行的方法,用于基于PCR的常规呼吸道病毒检测在儿童群体.
- 这种方法可以改善儿科呼吸道诊断中的患者舒适性和遵从性.
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