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通过祖先序列重建来增强Laccase的基质适应性,用于真菌毒素排毒中的应用

Shuaifeng Liu^1,2, Shenglong Liu^1,2, Yaqi Zhang³

¹Anhui Key Laboratory of Biocatalysis and Modern Biomanufacturing, Key Laboratory of Enzymatic In Vitro Diagnostic Technology of Anhui Province, School of Life Sciences, Anhui University, Hefei, Anhui 230000, China.

Journal of agricultural and food chemistry

|November 12, 2025

概括

改造的真菌乳糖酶 LacANC278有效降解了谷物中的有毒的亚拉托克辛B1 (AFB1) 和泽拉伦 (ZEN). 这种增强的酶表现出更好的稳定性和更高的转化率,为真菌毒素排毒提供了一个有前途的解决方案.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
食品安全食品安全

背景情况:

污染了菌毒素的成粒,如阿弗拉托克辛B1 (AFB1) 和泽拉伦 (ZEN),对健康构成严重危害.
由Coprinopsis cinerea*的LCC5所示的真菌乳酸酶正在被探索它们的真菌毒素降解能力.

研究的目的:

设计一种具有增强活性和稳定性的真菌乳酶变体,用于降解AFB1和ZEN.
为了识别和表征一种在真菌毒素转化中表现优异的新型祖先乳糖酶.

主要方法:

使用祖先序列重建来创建LCC5变体.
进行了分子对接,以确定有希望的祖先乳糖酶候选者.
评估了玉米粉中的AFB1和ZEN的酶活性,稳定性和转化效率.
临时确定了转化产品,并评估了它们的安全性.

主要成果:

与野生类型LCC5.5相比,工程化祖先乳糖酶,LacANC278,对AFB1 (23%的增加) 和ZEN (61%的增加) 的转化率显著更高.
LacANC278的半衰期增加了四倍 (16小时与4小时相比).
在玉米粉中,LacANC278在最佳条件下实现了88%的AFB1减少和89%的ZEN减少,其中AFQ1和2-OH-ZEN被确定为主要产品.

结论:

祖先序列重建是一种有效的策略,用于增强真菌乳酶对抗菌毒素的性能.
LacANC278是一种强大的生物催化剂,用于农产品中AFB1和ZEN的排毒.
对转化产品的安全性进行进一步调查是有必要的.

关键词:

非洲毒素B1是什么?祖先的序列重建的重建.排毒排毒排毒排毒拉卡斯拉卡斯拉卡斯拉卡斯拉卡斯拉卡斯菌类毒素 (mycotoxin) 是一种有毒的物质.没有一个Zearalenone

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