Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Enhancing Dose Efficiency of Optimum Bright-Field Scanning Transmission Electron Microscopy Using a Phase-Shifted Electron Probe.

Small methods·2026
Same author

Direct observation of cation-dependent polarisation switching dynamics in fluorite ferroelectrics.

Nature communications·2026
Same author

A Bayesian approach to spectroscopic depth sectioning for locating dopant atoms.

Journal of microscopy·2026
Same author

Locating the atoms at the hard-soft interface of gold nanoparticles.

Nature communications·2026
Same author

Dopant Site Occupancy Determined by Core-Loss-Filtered, Position-Averaged Convergent Beam Electron Diffraction.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2025
Same author

Quantitative Structure Determination from Experimental Four-Dimensional Scanning Transmission Electron Microscopy via the Scattering Matrix.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2025
Same journal

Gradient-Based Experimental Design for Defect Detection in MoS2 Including Emission Potentials for Thermal Diffuse Scattering.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

An Automated Atom Probe Tomography Cluster Detection Approach Using Transfer Learning.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Correlative Light and Electron Microscopy Visualization of Helicobacter pylori in Human Saliva.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Integrating Morpho-Anatomy and Histochemistry to Characterize Native Brazilian Eugenia Species.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Polyalthia Longifolia Induced Apoptosis via miR-484 Downregulation: A Multimodal In Situ Microscopy, In Vitro, and In Vivo Investigation.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Rhythmic Pattern of the Ovarian Development in Posthatching Japanese Quail (Coturnix coturnix japonica): Histological, Ultrastructural, and Immunohistochemical Study.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
查看所有相关文章

相关实验视频

Updated: Jan 6, 2026

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

5.3K

从四维扫描传输电子显微镜通过散射矩阵优化结构因素.

Kousuke Ooe1,2, Alireza Sadri1, Scott D Findlay1

  • 1School of Physics and Astronomy, Monash University, Wellington Rd, Clayton, Victoria 3800, Australia.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|November 12, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了一种新的算法,用于扫描传输电子显微镜 (STEM) 中的定量潜力检索. 这种方法可以准确地从更厚的样本中重建材料特性,克服多个散射挑战.

关键词:
在4D-STEM中,在OBF的声音.扫描传输电子显微镜扫描传输电子显微镜散射矩阵是一个散射矩阵.

更多相关视频

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

9.8K
Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

880

相关实验视频

Last Updated: Jan 6, 2026

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

5.3K
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

9.8K
Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

880

科学领域:

  • 材料科学 材料科学 材料科学
  • 凝聚物质物理学 凝聚物质物理学
  • 电子显微镜电子显微镜

背景情况:

  • 扫描传输电子显微镜 (STEM) 中的定量潜力检索对于材料表征至关重要.
  • 较厚的样本由于显著的多重散射效应而存在挑战.

研究的目的:

  • 开发一种基于梯度下降的新型优化算法,用于从4D STEM中的厚样本中检索定量潜力.
  • 为了克服电子显微镜中多重散射所带来的局限性.

主要方法:

  • 使用散射矩阵 (S-矩阵) 来建模多重散射效应.
  • 在没有常见规范化术语的情况下使用梯度下降优化算法.
  • 使用最佳亮场STEM数据作为初始潜在猜测和基于测量/估计的4DSTEM数据的损失函数.

主要成果:

  • 在各种模拟条件 (厚度,剂量,不一致性) 中,成功地从厚样本中检索出定量潜力.
  • 在实验4D STEM数据上证明了算法的有效性.
  • 研究了对细分探测器算法的扩展.

结论:

  • 提出的梯度下降优化算法有效地使得在STEM中对更厚的样本进行定量潜力检索.
  • 这种方法通过减轻多个散射工件,提高了材料表征能力.