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作为对低CO2压力和m6的反应而进行的Epitranscriptomic重编程一种工程来增强Nannochloropsis oceanica中的生物质生产

Han Zhu^1,2, Yikai Wu^1,2, Meng Feng^1,2

¹Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, 571158, China.

The Plant journal : for cell and molecular biology

|November 12, 2025

概括

在Nannochloropsis oceanica中的N-甲基氨酸 (m6A) RNA修饰在低CO2下动态重编程. 工程m6A增加了应力耐受性和生物量,为微藻表观遗传学铺平了道路.

科学领域:

史诗转录组学史诗转录组学
分子生物学分子生物学
微藻生物技术微藻生物技术

背景情况:

N-甲基氨酸 (m6A) 是真核生物中最丰富的RNA修饰,调节基因表达.
微藻中m6A的作用和动态,特别是在环境压力下,在很大程度上是未被探索的.
海洋纳米藻 (Nannochloropsis oceanica) 是一种具有重要的生物技术潜力的模型微藻.

研究的目的:

为了调查m6ARNA修饰的动态变化和重编程m6ARNA修饰在Nannochloropsis oceanica在低二氧化碳条件的反应.
阐明m6A甲基酶 (NoMTA和NoMTB) 在调节基因表达和细胞对低CO2反应中的功能.
设计m6A修改以提高低CO2压力耐受性和北大洋洲生物质生产率.

主要方法:

甲基化RNA免疫沉测序 (m6A-IP-Seq) 用于绘制m6A峰值的地图.
基因组编辑 (CRISPR/Cas9) 来产生m6A甲基酶 (NoMTA,NoMTB) 的淘汰突变.
CRISPR/dCas13系统用于工程 m6A 修改.

主要成果:

m6A峰值主要丰富在N. oceanica转录的3'UTR中.
观察到m6A丰富度与对CO2敏感基因的转录之间存在正相关性.
淘汰NoMTA和NoMTB导致m6A重编程,改变碳代谢和光呼吸基因的表达,并减少生长.
使用CRISPR/dCas13进行的工程m6A修改提高了低CO2压力耐受性和生物质生产率.

结论:

海洋表现出动态的m6A重编程作为一种多功能策略,以应对低二氧化碳压力.
在低二氧化碳条件下,m6A甲基酶NoMTA和NoMTB在调节基因表达和细胞适应性方面发挥着至关重要的作用.
工程 m6A 修改为提高工业应用中的微藻性能提供了一个有希望的途径,特别是在二氧化碳有限的环境下.

关键词:

这就是MeRIP-序列化.海洋中的纳米化物 (Nanochloropsis oceanica) 是一种 m6A 工程工程师 m6A 修改的修改 m6A作家是一个作家.

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