兴申洁可以通过PI3K/Akt路径调节proBDNF/BDNF信号传递来改善中风后抑郁症
Xiang Chen1, Wei Zhao2, Zhenzhen Hou3
1Department of Neurology and Rehabilitation, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, 200137, China; Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong, China.
Journal of ethnopharmacology
|November 12, 2025
概括
兴申洁脱剂 (XSJY) 通过减少海马细胞亡并通过PI3K/Akt和BDNF通路恢复神经营养平衡,减轻大鼠中风后抑郁症 (PSD).
科学领域:
- 神经科学是一个神经科学.
- 药理学 药理学是指药理学的学科.
- 传统中国医药 传统中国医药
背景情况:
- 兴申洁宇 (XSJY) 是一种传统的中国草药配方,用于治疗中风后抑郁症 (PSD).
- 它在PSD中的神经保护和抗抑郁机制仍然不清楚.
- 对于PSD,XSJY的临床应用需要机械学的理解.
研究的目的:
- 在PSD的老鼠模型中研究XSJY的抗抑郁作用.
- 阐明涉及亡调节和PI3K/Akt/proBDNF/BDNF信号通路的分子机制.
- 确定PI3K/Akt通路在XSJY治疗作用中的作用.
主要方法:
- 使用中脑动脉阻塞 (MCAO) 和慢性不可预测的轻度压力 (CUMS) 建立了一个PSD大鼠模型.
- 使用HPLC/ESI-MS.描述了XSJY的化学特征.
- 服用低/高剂量的XSJY或塞特拉林,评估行为结果,海马细胞亡 (TUNEL) 和关键信号分子的蛋白质表达 (西方抹杀).
- 利用PI3K抑制剂LY294002来确认路径的特异性.
主要成果:
- XSJY显著改善了类似抑郁症的行为,并减少了海马细胞亡,特别是在牙状 (DG).
- XSJY上调了p-Akt,增强了BDNF/TrkB信号传递,并减少了proBDNF/p75NTR的表达.
- 抑制PI3K/Akt路径部分逆转了XSJY的影响,表明路径参与和补充机制.
结论:
- 在PSD大鼠中,XSJY通过减少海马细胞亡和恢复神经营养平衡来显示抗抑郁作用.
- 治疗作用部分通过PI3K/Akt和proBDNF/BDNF信号通路进行介导.
- XSJY表现出多目标的神经保护作用,表明复杂的潜在机制.
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