高血糖不同影响中性粒细胞在囊性纤维化和野生型支气管上皮质的传播
Guiying Cui1, Analía Vazquez Cegla1, Jonica Brown1
1Division of Pulmonology, Asthma, Cystic Fibrosis, and Sleep, Department of Pediatrics, Emory University School of Medicine, Emory + Children's Center for Cystic Fibrosis and Airways Disease Research, Children's Healthcare of Atlanta, USA.
概括
高血糖水平会使中性粒细胞 (PMN) 在囊性纤维化 (CF) 呼吸道中的运动恶化,但CFTR调节器疗法显示部分改善. 血糖状况和CFTR基因型在CF肺部疾病中影响PMN转移.
科学领域:
- 肺部医学 肺部医学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 囊性纤维化 (CF) 肺部疾病涉及慢性炎症和过度的中性粒细胞 (PMN) 流入呼吸道.
- 与CF相关的糖尿病 (CFRD) 加剧了气道炎症和PMN透.
研究的目的:
- 为了研究血糖状态 (正常与高葡萄糖) 对中性粒细胞通过呼吸道上皮的传播的影响.
- 确定高效调节器疗法 (HEMT) 是否可以影响CF模型中的PMN转移率.
主要方法:
- 在正常葡萄糖 (NG) 和高葡萄糖 (HG) 条件下,通过支气管上皮细胞系 (16HBE-WT,16HBE-CF) 和初级细胞 (NhBE,CFhBE) 检查了PMN传播.
- 经过转移后评估的体抗性 (TER) 和PMN形态.
- 研究了HEMT对PMN转移的影响.
主要成果:
- 高血糖 (HG) 显著降低了体抵抗 (TER) 和增加了PMN在健康细胞之间传播 (16HBE-WT,NhBE).
- PMN转移率受到葡萄糖状况和CFTR基因型 (F508del-CFTR) 的影响.
- 转移的PMN表现出形态变化,HEMT部分正常化了转移率.
结论:
- 高葡萄糖条件显著影响PMN通过呼吸道上皮的传播.
- 表皮细胞的CFTR基因型对转移的PMN产生差异性影响.
- 在CF中,HEMT在调节PMN转移方面显示出潜力,这表明了治疗途径.
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